Recombinant Arabidopsis thaliana 3-hydroxyisobutyryl-CoA hydrolase 1 (CHY1)

Code CSB-YP885369DOA
MSDS
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Source Yeast
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Code CSB-EP885369DOA
MSDS
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Source E.coli
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Code CSB-EP885369DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885369DOA
MSDS
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Source Baculovirus
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Code CSB-MP885369DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CHY1
Uniprot No.
Alternative Names
CHY1; At5g65940; K14B20.113-hydroxyisobutyryl-CoA hydrolase 1; EC 3.1.2.-; EC 3.1.2.4; CoA-thioester hydrolase CHY1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-378
Target Protein Sequence
MAVEMASQSQ VLVEEKSSVR ILTLNRPKQL NALSFHMISR LLQLFLAFEE DPSVKLVILK GHGRAFCAGG DVAAVVRDIN QGNWRLGANY FSSEYMLNYV MATYSKAQVS ILNGIVMGGG AGVSVHGRFR IATENTVFAM PETALGLFPD VGASYFLSRL PGFFGEYVGL TGARLDGAEM LACGLATHFV PSTRLTALEA DLCRINSNDP TFASTILDAY TQHPRLKQQS AYRRLDVIDR CFSRRTVEEI ISALEREATQ EADGWISATI QALKKGSPAS LKISLRSIRE GRLQGVGQCL IREYRMVCHV MKGEISKDFV EGCRAILVDK DKNPKWEPRR LEDMKDSMVE QYFERVERED DLKLPPRNNL PALGIAKL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in valine catabolism. May be indirectly involved in benzoic acid biosynthesis and in cold signaling and cold tolerance.
Gene References into Functions
  1. Findings suggest that at least a part of benzoic acid biosynthesis occurs in the peroxisomes, although the specific pathway that leads to benzoic acid and the specific biochemical role of Chy1 remain unclear. PMID: 19538395
  2. CHY1 encodes an enzyme acting in valine catabolism; seedlings deficient in CHY1 are sucrose-dependent during germination, probably because accumulation of a valine catabolic intermediate blocks fatty acid beta-oxidation. PMID: 11404361
Subcellular Location
Peroxisome.
Protein Families
Enoyl-CoA hydratase/isomerase family
Tissue Specificity
Expressed in roots, leaves, flowers and siliques.
Database Links

KEGG: ath:AT5G65940

STRING: 3702.AT5G65940.1

UniGene: At.9496

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