Recombinant Arabidopsis thaliana 3-isopropylmalate dehydrogenase 1, chloroplastic (IMDH1)

Code CSB-YP874362DOA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP874362DOA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP874362DOA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP874362DOA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP874362DOA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
IMDH1
Uniprot No.
Alternative Names
IMDH3; IMD3; IPMDH3; At1g31180; F28K20.143-isopropylmalate dehydrogenase 3; chloroplastic; 3-IPM-DH 3; AtIMDH2; AtIMDH3; IMDH 3; EC 1.1.1.85; Beta-IPM dehydrogenase 3; Isopropylmalate dehydrogenase 3; AtIMD3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
35-404
Target Protein Sequence
AAASPV KKRYNITLLP GDGIGPEVIS VAKNVLQKAG FLQGLEFDFQ EMPFGGAALD LVGVPLPEET STAAKQSDAI LLGAIGGYKW DKNEKHLRPE MGLLNIRRDL NVFANLRPAT VLPQLVDAST LKKEVAQGVD MMIVRELTGG IYFGEPRGIT INENGEEVGF NTEIYAAHEI DRIARVAFET ARKRRGKLCS VDKANVLDAS ILWRKRVTAL ASEYPDVELS HMYVDNAAMQ LVRDPKQFDT IVTNNIFGDI LSDEASMITG SIGMLPSASL GESGPGLFEP IHGSAPDIAG QDKANPLATI LSAAMLLKYG LGEEKAAKMI EDAVVDALNK GFRTGDIYSP GNKLVGCKEM GEEVLKSVDS KVPV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Involved in leucine biosynthesis; catalyzes the oxidative decarboxylation step in leucine biosynthesis (primary metabolism). Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate, 3-IPM) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Gene References into Functions
  1. IPMDH2 and IPMDH3 primarily function in leucine biosynthesis, are essential for pollen development and are needed for proper embryo sac development. PMID: 20840499
  2. IPMDH is involved in leucine biosynthetic pathway. Three IPMDH ORF sequences designated as AtIMD1, AtIMD2, and AtIMD3 are localized in plastids. AtIMD3 transcripts were found mainly in flowers and cauline leaves. [AtIMD3; IPMDH] PMID: 15849421
Subcellular Location
Plastid, chloroplast stroma.
Protein Families
Isocitrate and isopropylmalate dehydrogenases family
Tissue Specificity
Mostly expressed constitutively at high levels in seedlings, cotyledons, hypocotyls, flowers, pollen and leaves and, to a lower extent, in roots and stems.
Database Links

KEGG: ath:AT1G31180

STRING: 3702.AT1G31180.1

UniGene: At.40403

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1