Recombinant Arabidopsis thaliana 3-ketoacyl-CoA thiolase 2, peroxisomal (PED1)

Code CSB-YP701126DOA
MSDS
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Source Yeast
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Code CSB-EP701126DOA
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Source E.coli
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Code CSB-EP701126DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP701126DOA
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Source Baculovirus
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Code CSB-MP701126DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PED1
Uniprot No.
Alternative Names
PED1; KAT2; At2g33150; F25I18.11; 3-ketoacyl-CoA thiolase 2; peroxisomal; EC 2.3.1.16; Acetyl-CoA acyltransferase 2; Beta-ketothiolase 2; Peroxisomal 3-oxoacyl-CoA thiolase 2; Peroxisome defective protein 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
35-462
Target Protein Sequence
LAGDSA AYQRTSLYGD DVVIVAAHRT PLCKSKRGNF KDTYPDDLLA PVLRALIEKT NLNPSEVGDI VVGTVLAPGS QRASECRMAA FYAGFPETVA VRTVNRQCSS GLQAVADVAA AIKAGFYDIG IGAGLESMTT NPMAWEGSVN PAVKKFAQAQ NCLLPMGVTS ENVAQRFGVS RQEQDQAAVD SHRKAAAATA AGKFKDEIIP VKTKLVDPKT GDEKPITVSV DDGIRPTTTL ASLGKLKPVF KKDGTTTAGN SSQVSDGAGA VLLMKRSVAM QKGLPVLGVF RTFAAVGVDP AIMGIGPAVA IPAAVKAAGL ELDDIDLFEI NEAFASQFVY CRNKLGLDPE KINVNGGAMA IGHPLGATGA RCVATLLHEM KRRGKDCRFG VVSMCIGTGM GAAAVFERGD GVDELRNARK VEAQGLLSKD AR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in long chain fatty-acid beta-oxidation prior to gluconeogenesis during germination and subsequent seedling growth. Confers sensitivity to 2,4-dichlorophenoxybutiric acid (2,4-DB). Required for local and systemic induction of jasmonic acid (JA) biosynthesis after wounding. Seems to be involved in JA biosynthesis during senescence.
Gene References into Functions
  1. Peroxisomal KAT2 and KAT5 have partially redundant functions and operate downstream of AIM1 to provide beta-oxidation functions essential for inflorescence development and fertility. PMID: 25297549
  2. Mutations within the 3-ketoacyl-CoA thiolase 2 (KAT2) gene interferes with the balance between silique development and the continued initiation of floral meristems. [KAT2] PMID: 17728299
  3. KAT2 as an essential component for the timely onset of leaf senescence in Arabidopsis. PMID: 18441338
Subcellular Location
Peroxisome. Glyoxysome. Note=Peroxisomal targeting signal type 2 (PTS2-type) import is temperature and ATP-dependent, induced by zinc ions and PTS1-type import, but repressed by mature forms of PED1.
Protein Families
Thiolase family
Tissue Specificity
Accumulates in etiolated cotyledons and in seedlings, also present in roots, flowers and siliques (at protein level). High levels in wounded leaves.
Database Links

KEGG: ath:AT2G33150

STRING: 3702.AT2G33150.1

UniGene: At.23661

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