Recombinant Arabidopsis thaliana 3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic (KAS2)

Code CSB-YP887250DOA
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Source Yeast
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Code CSB-EP887250DOA
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Source E.coli
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Code CSB-EP887250DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887250DOA
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Source Baculovirus
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Code CSB-MP887250DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KAS2
Uniprot No.
Alternative Names
KAS2; FAB1; At1g74960; F25A4.7; F9E10.19; 3-oxoacyl-[acyl-carrier-protein] synthase II; chloroplastic; EC 2.3.1.41; Beta-ketoacyl-acyl-carrier-protein synthase II; AtKAS2; Beta-ketoacyl-ACP synthetase 2; Protein FATTY ACID BIOSYNTHESIS 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
104-541
Target Protein Sequence
ASSGGAM AVMEMEKEAA VNKKPPTEQR RVVVTGMGVE TSLGHDPHTF YENLLQGNSG ISQIENFDCS EFPTRIAGEI KSFSTEGWVA PKLSKRMDKF MLYLLTAGKK ALADGGVTDE VMAEFDKTKC GVLIGSAMGG MKVFYDAIEA LRISYKKMNP FCVPFATTNM GSAMLAMDLG WMGPNYSIST ACATSNFCIL NSANHIIKGE ADVMLCGGSD AVIIPIGLGG FVACRALSQR NNDPTKASRP WDTNRDGFVM GEGAGVLLLE ELEHAKKRGA TIYAEFLGGS FTCDAYHMTE PHPDGAGVIL CIERALASAG ISKEQINYIN AHATSTHAGD IKEYQALAHC FGQNPELKVN STKSMIGHLL GAAGAVEAVA TVQAIRTGWV HPNINLENPD SGVDTKLLVG PKKERLDIKA ALSNSFGFGG HNSSIIFAPY K
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential protein that catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-16 and C-16 to unsaturated C-18 fatty acids. Confers resistance to low temperatures by maintaining chloroplast membranes integrity. Involved in the regulation of fatty acids ratios during seed metabolism. Required for embryo development, especially at the transition from the globular to the heart stage.
Gene References into Functions
  1. the identification of two suppressor mutations, one in act1, which encodes the chloroplast acyl-ACP:glycerol-3-phosphate acyltransferase, and one in lpat1, which encodes the chloroplast acyl-ACP:lysophosphatidic acid acyltransferase, is reported.. PMID: 26224803
  2. The suppressor mutation of fab1 was identified, by map-based and candidate-gene approaches, as a hypomorphic allele of lysophosphatidic acid acyltransferase1 (lpat1), lpat1-3. PMID: 20488893
  3. A change in shape of the major thylakoid lipid, monogalactosyldiacylglycerol, mediated by the fad5-2 mutation, may compensate for changes in lipid structure resulting from the original fab1 mutation. PMID: 16698898
  4. Results suggest that AtKAS2 is required for embryo development in Arabidopsis during the transition from the globular to the heart stage. PMID: 18506098
Subcellular Location
Plastid, chloroplast stroma.
Protein Families
Beta-ketoacyl-ACP synthases family
Tissue Specificity
Mostly expressed in siliques, and, to a lower extent, in leaves, stems, flower buds, and flowers.
Database Links

KEGG: ath:AT1G74960

STRING: 3702.AT1G74960.1

UniGene: At.26097

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