Recombinant Arabidopsis thaliana 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial (KAS)

Code CSB-YP809239DOA
MSDS
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Source Yeast
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Code CSB-EP809239DOA
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Source E.coli
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Code CSB-EP809239DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP809239DOA
MSDS
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Source Baculovirus
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Code CSB-MP809239DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KAS
Uniprot No.
Alternative Names
KAS; At2g04540; T1O3.5; 3-oxoacyl-[acyl-carrier-protein] synthase; mitochondrial; EC 2.3.1.41; Beta-ketoacyl-ACP synthase; mtKAS
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
29-461
Target Protein Sequence
SH RRVVVTGLGM VTPLGRGVET TWRRLIDGEC GIRGLTLDDL KMKSFDEETK LYTFDQLSSK VAAFVPYGSN PGEFDEALWL NSKAVANFIG YAVCAADEAL RDAEWLPTEE EEKERTGVSI GGGIGSICDI VEAAQLICEK RLRRLSPFFI PKILVNMASG HVSMKYGFQG PNHAAVTACA TGAHSIGDAT RMIQFGDADV MVAGGTESSI DALSVAGFSR SRALSTKFNS SPQEASRPFD CDRDGFVIGE GSGVIVLEEY EHAKRRGAKI YAELCGYGMS GDAHHITQPP EDGKGAVLAM TRALRQSGLC PNQIDYVNAH ATSTPIGDAV EARAIKTVFS EHATSGTLAF SSTKGATGHL LGAAGAVEAI FSILAIHHGV APMTLNVKNP DPIFDKRFMP LTTSKKMLVR TAMSNSFGFG GTNASLLFAS I
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes all the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Able to elongate saturated acyl chains from 4 to at least 16 carbons. Uses malonyl-CoA but not acetyl-CoA as primer substrate. When expressed in a heterologous system, reveals a bimodal distribution of products, with peaks at C8 and C14-C16. The major product of the reaction (octanoyl-ACP) is required for the lipoylation of essential mitochondrial proteins.
Gene References into Functions
  1. encodes beta-ketoacyl-[acyl carrier protein] synthase(mtKAS), but mitochondrial protein lipoylation does not exclusively depend on the mtKAS pathway of lipoate biosynthesis in leaves and may occur independently of this pathway in roots PMID: 17616510
Subcellular Location
Mitochondrion.
Protein Families
Beta-ketoacyl-ACP synthases family
Tissue Specificity
Expressed at the same level in leaves, roots and flowers.
Database Links

KEGG: ath:AT2G04540

STRING: 3702.AT2G04540.1

UniGene: At.41299

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