Recombinant Arabidopsis thaliana 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 1 (MTN1)

Code CSB-YP874580DOA
MSDS
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Source Yeast
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Code CSB-EP874580DOA
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Source E.coli
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Code CSB-EP874580DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874580DOA
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Source Baculovirus
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Code CSB-MP874580DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MTN1
Uniprot No.
Alternative Names
MTN1; MTAN; MTAN1; At4g38800; T9A14.80; 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase 1; AtMTN1; EC 3.2.2.9; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; MTA/SAH nucleosidase 1; AtMTAN1; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-267
Target Protein Sequence
MAPHGDGLSD IEEPEVDAQS EILRPISSVV FVIAMQAEAL PLVNKFGLSE TTDSPLGKGL PWVLYHGVHK DLRINVVCPG RDAALGIDSV GTVPASLITF ASIQALKPDI IINAGTCGGF KVKGANIGDV FLVSDVVFHD RRIPIPMFDL YGVGLRQAFS TPNLLKELNL KIGRLSTGDS LDMSTQDETL IIANDATLKD MEGAAVAYVA DLLKIPVVFL KAVTDLVDGD KPTAEEFLQN LTVVTAALEG TATKVINFIN GRNLSDL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Enzyme of the methionine cycle that catalyzes the irreversible cleavage of the glycosidic bond in 5'-methylthioadenosine (MTA) to adenine and 5'-methylthioribose. Contributes to the maintenance of AdoMet homeostasis and is required to sustain high rates of ethylene synthesis. Inactive towards S-adenosylhomocysteine (SAH/AdoHcy).
Gene References into Functions
  1. MTA as a crucial metabolite that acts as a regulatory link between the Yang cycle and polyamine biosynthesis and identifies MTA nucleosidase as a crucial enzyme of the Yang cycle. [MTN1] PMID: 20345605
  2. determined the high-resolution structure of 5'-methylthioadenosine nucleosidase from Arabidopsis thaliana (AtMTAN) PMID: 16909418
  3. AtMTAN1 was characterized kinetically. PMID: 18342331
  4. The ATMTN1 protein methylthioadenosine nucleosidase activity can be inhibited specifically by Ca(2+)-bound CBL3. PMID: 18945934
Protein Families
PNP/UDP phosphorylase family, MtnN subfamily
Tissue Specificity
Expressed in roots, leaves, stems, cauline leaves and flowers.
Database Links

KEGG: ath:AT4G38800

STRING: 3702.AT4G38800.1

UniGene: At.2827

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