Recombinant Arabidopsis thaliana 60S ribosomal protein L24-2 (RPL24B)

Code CSB-YP336475DOA
MSDS
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Source Yeast
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Code CSB-EP336475DOA
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Source E.coli
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Code CSB-EP336475DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP336475DOA
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Source Baculovirus
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Code CSB-MP336475DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RPL24B
Uniprot No.
Alternative Names
RPL24B; STV1; At3g53020; F8J2_190; 60S ribosomal protein L24-2; Protein SHORT VALVE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-163
Target Protein Sequence
MVLKTELCRF SGQKIYPGRG IRFIRSDSQV FLFLNSKCKR YFHNKLKPSK LAWTAMYRKQ HKKDAAQEAV KRRRRATKKP YSRSIVGATL EVIQKKRAEK PEVRDAAREA ALREIKERIK KTKDEKKAKK VEFASKQQKV KANFPKAAAA SKGPKVGGGG GKR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Might have an extraribosomal function in reinitiation of translation of ETTIN and MONOPTEROS genes that are involved in the auxin-mediated gynoecium patterning. Essential in leaf polarity establishment, probably having a role for translation in leaf dorsoventral patterning to specify leaf adaxial identity.
Gene References into Functions
  1. Data suggest that SHORT VALVE 1 (STV1) promotes miRNA biogenesis through facilitating the recruitment of pri-miRNAs to their processing complex. PMID: 28115696
  2. These data represent the first genome-wide analysis of translation in a eukaryote defective in the large ribosomal subunit. RPL24 and eIF3h play similar but non-identical roles in eukaryotic translation. PMID: 24377433
  3. In addition to the role of RPL24 in ribosome-related whole-plant growth and the development of several organs, RPL24 functions specifically in auxin-mediated apical-basal patterning of the gynoecium. [RPL24B] PMID: 16227452
Subcellular Location
Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm.
Protein Families
Eukaryotic ribosomal protein eL24 family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT3G53020

STRING: 3702.AT3G53020.1

UniGene: At.22369

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