Recombinant Arabidopsis thaliana AFP homolog 2 (AFPH2)

Code CSB-YP871178DOA
MSDS
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Source Yeast
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Code CSB-EP871178DOA
MSDS
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Source E.coli
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Code CSB-EP871178DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871178DOA
MSDS
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Source Baculovirus
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Code CSB-MP871178DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AFPH2
Uniprot No.
Alternative Names
AFPH2; NINJA; At4g28910; F25O24.30; AFP homolog 2; Novel interactor of JAZ
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-425
Target Protein Sequence
MDDDNGLELS LGLSCGGSTG KAKGNNNNNA GSSSENYRAE GGDRSAKVID DFKNFLHPTS QRPAEPSSGS QRSDSGQQPP QNFFNDLSKA PTTEAEASTK PLWVEDESRK EAGNKRKFGF PGMNDDKKKE KDSSHVDMHE KKTKASHVST ATDEGSTAEN EDVAESEVGG GSSSNHAKEV VRPPTDTNIV DNLTGQRRSN HGGSGTEEFT MRNMSYTVPF TVHPQNVVTS MPYSLPTKES GQHAAATSLL QPNANAGNLP IMFGYSPVQL PMLDKDGSGG IVALSQSPFA GRVPSNSATA KGEGKQPVAE EGSSEDASER PTGDNSNLNT AFSFDFSAIK PGMAADVKFG GSGARPNLPW VSTTGSGPHG RTISGVTYRY NANQIKIVCA CHGSHMSPEE FVRHASEEYV SPESSMGMTA ASAHT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as a transcriptional repressor. Negative regulator of jasmonate responses. Connects the JAZ proteins and the non-JAZ protein TIFY8 with the TOPLESS corepressors.
Gene References into Functions
  1. The role of NINJA in the root is to repress jasmonate signaling and allow normal cell elongation. PMID: 24003128
  2. Both NINJA and TPL were shown to function as negative regulators of jasmonate signaling. PMID: 20671423
  3. Arabidopsis JAZ proteins recruit the Groucho/Tup1-type co-repressor TOPLESS (TPL) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). [NINJA] PMID: 20360743
Subcellular Location
Nucleus.
Protein Families
Ninja family
Database Links

KEGG: ath:AT4G28910

STRING: 3702.AT4G28910.1

UniGene: At.3398

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