Recombinant Arabidopsis thaliana AP2/ERF and B3 domain-containing transcription repressor TEM1 (TEM1)

Code CSB-YP880196DOA
MSDS
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Source Yeast
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Code CSB-EP880196DOA
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Source E.coli
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Code CSB-EP880196DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880196DOA
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Source Baculovirus
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Code CSB-MP880196DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TEM1
Uniprot No.
Alternative Names
TEM1; At1g25560; F2J7.3; AP2/ERF and B3 domain-containing transcription repressor TEM1; Protein TEMPRANILLO 1; RAV1-like ethylene-responsive transcription factor TEM1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-361
Target Protein Sequence
MEYSCVDDSS TTSESLSIST TPKPTTTTEK KLSSPPATSM RLYRMGSGGS SVVLDSENGV ETESRKLPSS KYKGVVPQPN GRWGAQIYEK HQRVWLGTFN EEEEAASSYD IAVRRFRGRD AVTNFKSQVD GNDAESAFLD AHSKAEIVDM LRKHTYADEF EQSRRKFVNG DGKRSGLETA TYGNDAVLRA REVLFEKTVT PSDVGKLNRL VIPKQHAEKH FPLPAMTTAM GMNPSPTKGV LINLEDRTGK VWRFRYSYWN SSQSYVLTKG WSRFVKEKNL RAGDVVCFER STGPDRQLYI HWKVRSSPVQ TVVRLFGVNI FNVSNEKPND VAVECVGKKR SREDDLFSLG CSKKQAIINI L
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional repressor of flowering time on long day plants. Acts directly on FT expression by binding 5'-CAACA-3' and 5'-CACCTG-3 sequences. Functionally redundant with TEM2.
Gene References into Functions
  1. Data show that TEMPRANILLO (TEM) acts as a link between gibberellins (GAs) and cytokinin signaling in the epidermis by negatively regulating downstream genes of both trichome formation pathways. PMID: 26802039
  2. TEMPRANILLO genes link photoperiod and gibberellin pathways to control flowering. PMID: 22549837
  3. TEMPRANILLO genes (TEM1 and TEM2) act as novel direct Flowering locus T repressors. PMID: 18718758
Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, RAV subfamily
Tissue Specificity
Expressed in leaves.
Database Links

KEGG: ath:AT1G25560

STRING: 3702.AT1G25560.1

UniGene: At.17318

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