Recombinant Arabidopsis thaliana Abscisic acid receptor PYL4 (PYL4)

Code CSB-YP530515DOA
MSDS
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Source Yeast
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Code CSB-EP530515DOA
MSDS
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Source E.coli
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Code CSB-EP530515DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP530515DOA
MSDS
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Source Baculovirus
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Code CSB-MP530515DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PYL4
Uniprot No.
Alternative Names
PYL4; ABIP2; RCAR10; At2g38310; T19C21.20; Abscisic acid receptor PYL4; ABI1-binding protein 2; PYR1-like protein 4; Regulatory components of ABA receptor 10
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-207
Target Protein Sequence
MLAVHRPSSA VSDGDSVQIP MMIASFQKRF PSLSRDSTAA RFHTHEVGPN QCCSAVIQEI SAPISTVWSV VRRFDNPQAY KHFLKSCSVI GGDGDNVGSL RQVHVVSGLP AASSTERLDI LDDERHVISF SVVGGDHRLS NYRSVTTLHP SPISGTVVVE SYVVDVPPGN TKEETCDFVD VIVRCNLQSL AKIAENTAAE SKKKMSL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA. Can be activated by both (-)-ABA and (+)-ABA.
Gene References into Functions
  1. Data indicate that bscisic acid (ABA) signaling is repressed by group A type 2C protein phosphatases (PP2Cs), and then ABA binds to its receptor of the ACTIN RESISTANCE1 (PYR1), PYR1-LIKE (PYL), and REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) family, which, in turn, inhibits PP2Cs and activates downstream ABA signaling. PMID: 28193765
  2. RSL1-PYL4 and RSL1-PYR1 interaction is localized to plasma membrane. RSL1 promoted PYL4 and PYR1 degradation in vivo and mediated in vitro ubiquitylation of the receptors. PMID: 25330042
  3. PYL4 A194T mutation enhances drought resistance through an interaction with PP2CA. PMID: 23864556
  4. Jasmonate signaling involves the abscisic acid receptor PYL4 to regulate metabolic reprogramming in Arabidopsis and tobacco. PMID: 21436041
Subcellular Location
Cytoplasm. Nucleus. Cell membrane. Vacuole.
Protein Families
PYR/PYL/RCAR abscisic acid intracellular receptor family
Database Links

KEGG: ath:AT2G38310

STRING: 3702.AT2G38310.1

UniGene: At.12775

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