Recombinant Arabidopsis thaliana Abscisic acid receptor PYR1 (PYR1),Biotinylated

Code CSB-EP527416DOA-B
Abbreviation Recombinant Mouse-ear cress PYR1 protein, Biotinylated
MSDS
Size $554
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
PYR1
Uniprot No.
Research Area
Others
Species
Arabidopsis thaliana (Mouse-ear cress)
Source
E.coli
Expression Region
1-191aa
Target Protein Sequence
MPSELTPEERSELKNSIAEFHTYQLDPGSCSSLHAQRIHAPPELVWSIVRRFDKPQTYKHFIKSCSVEQNFEMRVGCTRDVIVISGLPANTSTERLDILDDERRVTGFSIIGGEHRLTNYKSVTTVHRFEKENRIWTVVLESYVVDMPEGNSEDDTRMFADTVVKLNLQKLATVAEAMARNSGDGSGSQVT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
69.3 kDa
Protein Length
Full Length
Tag Info
N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA. Can be activated by both (-)-ABA and (+)-ABA. Promotes drought tolerance.
Gene References into Functions
  1. study suggests both nuclear and cytoplasmic PYR1 participate in the control of ABA signal transduction PMID: 28109881
  2. The transgenic plants show an acute abscisic acid (ABA) insensitivity, demonstrating the importance of ABA-mediated PYR/PYL/RCAR-PP2C contacts for receptor function in vivo. PMID: 21357183
  3. Pyr1 receptor contributes to ozone, reduced air humidity and darkness induced stomatal regulation. PMID: 23703845
  4. findings are generally consistent with the competitive-interaction model for PYR1 but highlight dynamical contributions of the PYR1 structure in mediating interaction selectivity PMID: 23825939
  5. This work illustrates how oligomerization of abscisic acid receptors can modulate hormonal responses in Arabidopsis and more generally, the sensitivity of a ligand-dependent signalling system. PMID: 21847091
  6. Subtle changes in the binding pockets of PYR/PYL proteins control ligand orientation inside the pocket, and this is sufficient to explain whether pyrabactin acts as an effective receptor agonist. [PYR1] PMID: 20729860
  7. X-ray data is reported for PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 in the presence of (+)-abscisic acid. The three proteins crystallize in space groups P3(1)21/P3(2)21, P2 and P1, respectively. X-ray intensity data were collected to 1.9-2.6 A resolution. PMID: 20383021
  8. findings show that abscissic binds to PYRABACTIN RESISTANCE 1 (PYR1),At4g17870, which in turn binds to and inhibits Type 2C protein phosphatases (HAB1) PMID: 19407142
  9. the crystal structure of Arabidopsis thaliana PYR1, which consists of a dimer in which one of the subunits is bound to ABA. PMID: 19898494
  10. structure of pyrabactin resistance 1 (PYR1); structure reveals an alpha/beta helix-grip fold & homodimeric assembly; abscisic acid (ABA) binding within a large internal cavity switches structural motifs distinguishing ABA-free from ABA-bound conformations PMID: 19933100

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Subcellular Location
Cytoplasm. Cytoplasm, cytosol. Nucleus. Cell membrane. Vacuole.
Protein Families
PYR/PYL/RCAR abscisic acid intracellular receptor family
Database Links

KEGG: ath:AT4G17870

STRING: 3702.AT4G17870.1

UniGene: At.2045

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