Recombinant Arabidopsis thaliana Actin-8 (ACT8)

Code CSB-YP839246DOA
MSDS
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Source Yeast
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Code CSB-EP839246DOA
MSDS
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Source E.coli
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Code CSB-EP839246DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839246DOA
MSDS
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Source Baculovirus
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Code CSB-MP839246DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ACT8
Uniprot No.
Alternative Names
ACT8; At1g49240; F27J15.1Actin-8
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-377
Target Protein Sequence
MADADDIQPI VCDNGTGMVK AGFAGDDAPR AVFPSVVGRP RHHGVMVGMN QKDAYVGDEA QSKRGILTLK YPIEHGVVSN WDDMEKIWHH TFYNELRIAP EEHPVLLTEA PLNPKANREK MTQIMFETFN SPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG FSLPHAILRL DLAGRDLTDY LMKILTERGY MFTTTAEREI VRDIKEKLSF VAVDYEQEME TSKTSSSIEK NYELPDGQVI TIGAERFRCP EVLFQPSFVG MEAAGIHETT YNSIMKCDVD IRKDLYGNIV LSGGTTMFSG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIS KAEYDEAGPG IVHRKCF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the vegetative actins.
Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Actin family
Tissue Specificity
Strongly expressed in nearly all vegetative tissues, and levels remain high in older tissues. Little or no expression is detected in mature pollen sacs, ovules, embryos or seeds.
Database Links

KEGG: ath:AT1G49240

STRING: 3702.AT1G49240.1

UniGene: At.24689

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