Recombinant Arabidopsis thaliana Acyl-CoA-binding domain-containing protein 1 (ACBP1), partial

Code CSB-YP879787DOA1
MSDS
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Source Yeast
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Code CSB-EP879787DOA1
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Source E.coli
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Code CSB-EP879787DOA1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP879787DOA1
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Source Baculovirus
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Code CSB-MP879787DOA1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ACBP1
Uniprot No.
Alternative Names
ACBP1; ACBP; At5g53470; MYN8.8; Acyl-CoA-binding domain-containing protein 1; Acyl-CoA binding protein 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds medium- and long-chain acyl-CoA esters with very high affinity. Can interact in vitro with arachidonyl-CoA, barely with oleoyl-CoA, but not with palmitoyl-CoA. Confers tolerance and binds to lead ions Pb(2+), probably by promoting lead translocation from roots to shoots. May function as an intracellular carrier of acyl-CoA esters.
Gene References into Functions
  1. ACBP1 appears to modulate the metabolism of two important lipid classes (fatty acids and sterols) influencing cellular signaling. PMID: 28500265
  2. Results suggest that ACBP1 participates in ABA-mediated seed germination and seedling development. PMID: 23448237
  3. ACBP1 overexpressors show alterations in phosphatidylcholine molecular species. Knockouts have enhanced freezing tolerance. PMID: 20107029
  4. When grown on Pb(II)-containing medium, transgenic Arabidopsis lines overexpressing ACBP1 were more tolerant to Pb(II)-induced stress than the wild type. PMID: 18182029
Subcellular Location
Cell membrane; Single-pass membrane protein. Secreted, cell wall. Endoplasmic reticulum membrane; Single-pass membrane protein. Note=Detected in the cell walls of the outer integument cells at the seed coat.
Protein Families
ACBP family
Tissue Specificity
Expressed at low levels in roots, stems, leaves, flowers, and siliques, especially within seeds.
Database Links

KEGG: ath:AT5G53470

STRING: 3702.AT5G53470.1

UniGene: At.8952

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