Recombinant Arabidopsis thaliana Agamous-like MADS-box protein AGL14 (AGL14)

Code CSB-YP654332DOA
MSDS
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Source Yeast
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Code CSB-EP654332DOA
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Source E.coli
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Code CSB-EP654332DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP654332DOA
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Source Baculovirus
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Code CSB-MP654332DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGL14
Uniprot No.
Alternative Names
AGL14; XAL2; At4g11880; T26M18.90Agamous-like MADS-box protein AGL14; Protein XAANTAL 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-221
Target Protein Sequence
MVRGKTEMKR IENATSRQVT FSKRRNGLLK KAFELSVLCD AEVALIIFSP RGKLYEFSSS SSIPKTVERY QKRIQDLGSN HKRNDNSQQS KDETYGLARK IEHLEISTRK MMGEGLDASS IEELQQLENQ LDRSLMKIRA KKYQLLREET EKLKEKERNL IAENKMLMEK CEMQGRGIIG RISSSSSTSE LDIDDNEMEV VTDLFIGPPE TRHFKKFPPS N
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that regulates root development by controlling meristem size and patterning of the root apical meristem. Regulates auxin transport and gradients in the root meristematic cells via direct regulation of the auxin efflux carrier PIN1 and PIN4 gene expression. Binds specifically to the CArG-box DNA sequences in the promoter regions of PIN1 and PIN4 genes. Involved in the regulation of shoot apical meristem (SAM) cell identities and transitions. Promotes flowering transition and participates in flower meristem maintenance and determinacy. Positively regulates TFL1 and WUS expression. Binds directly to the TFL1 regulatory sequences.
Gene References into Functions
  1. XAANTAL2 (XAL2/AGL14) is necessary and sufficient to induce flowering, and its regulation is important in floral meristem maintenance and determinacy. PMID: 25636918
  2. The MADS transcription factor XAL2/AGL14 modulates auxin transport during Arabidopsis root development by regulating PIN expression. PMID: 24121311
Subcellular Location
Nucleus.
Tissue Specificity
Preferentially expressed in roots. Expressed in lateral root cap, root epidermis, root endodermis, columella of the root meristematic region, the vascular cylinder in differentiated zones of the primary root and in emerged lateral root primordia. Expresse
Database Links

KEGG: ath:AT4G11880

STRING: 3702.AT4G11880.1

UniGene: At.42992

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