Recombinant Arabidopsis thaliana Agamous-like MADS-box protein AGL5 (AGL5)

Code CSB-YP329904DOA
MSDS
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Source Yeast
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Code CSB-EP329904DOA
MSDS
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Source E.coli
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Code CSB-EP329904DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP329904DOA
MSDS
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Source Baculovirus
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Code CSB-MP329904DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGL5
Uniprot No.
Alternative Names
AGL5; SHP2; At2g42830; F7D19.17; Agamous-like MADS-box protein AGL5; Protein SHATTERPROOF 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-246
Target Protein Sequence
MEGGASNEVA ESSKKIGRGK IEIKRIENTT NRQVTFCKRR NGLLKKAYEL SVLCDAEVAL VIFSTRGRLY EYANNSVRGT IERYKKACSD AVNPPTITEA NTQYYQQEAS KLRRQIRDIQ NLNRHILGES LGSLNFKELK NLESRLEKGI SRVRSKKHEM LVAEIEYMQK REIELQNDNM YLRSKITERT GLQQQESSVI HQGTVYESGV TSSHQSGQYN RNYIAVNLLE PNQNSSNQDQ PPLQLV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable transcription factor. Interacts genetically with TT16/AGL32 in a partially antagonistic manner during flower development. Is essential for the coordination of cell divisions in ovule, seed coat development and endosperm formation.
Gene References into Functions
  1. Gab1/SHP2/p38MAPK signaling pathway and Ang-2 have an essential role in regulating thrombin-induced monocyte adhesion and vascular leakage PMID: 27241812
  2. Study identified new roles for SHP1 and SHP2: they are required for synchronized ovule maturation and subsequent fertilization/seed maturation, seed mucilage production, and have an inhibitory effect which is antagonistic to the ABS function in controlling formative divisions and cell shape of the inner integument and seed coat layers. PMID: 27776173
  3. The stk shp1 shp2 ant quadruple mutant ovule lacks integument development as in ant single mutant. PMID: 20041269
  4. SHP1/2 and TAGL1, while distinct in molecular function, regulate similar activities via their necessity for seed dispersal in Arabidopsis and tomato, respectively. PMID: 19880793
  5. The present work identifies a new function for SHP1 and SHP2 genes in promoting stigma, style and medial tissue development. PMID: 19900437
  6. Bisindolylmaleimide I abolishes the FGF2-mediated association of Shp2 tyrosine phosphatase with Frs2 and Gab1. PMID: 17145761

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Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT2G42830

STRING: 3702.AT2G42830.2

UniGene: At.284

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