Recombinant Arabidopsis thaliana Anaphase-promoting complex subunit 11 (APC11)

Code CSB-YP868147DOA
MSDS
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Source Yeast
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Code CSB-EP868147DOA
MSDS
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Source E.coli
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Code CSB-EP868147DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP868147DOA
MSDS
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Source Baculovirus
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Code CSB-MP868147DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
APC11
Uniprot No.
Alternative Names
APC11; At3g05870; F10A16.17Anaphase-promoting complex subunit 11; Cyclosome subunit 11
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-84
Target Protein Sequence
MKVKILRWHA VASWTWDAQD ETCGICRMAF DGCCPDCKLP GDDCPLIWGA CNHAFHLHCI LKWVNSQTSQ AHCPMCRREW QFKE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex controls several key steps in the cell cycle by mediating ubiquitination and subsequent degradation of target proteins such as cyclins. The APC/C complex is required for the female gametophyte development and is involved in several aspect of development by controlling cell division and cell elongation. Involved in the control of endoreduplication. May recruit the E2 ubiquitin-conjugating enzymes to the complex.
Gene References into Functions
  1. APC/C-mediated cyclin B1 degradation is critical for cell cycle synchronization. PMID: 29424475
  2. Data indicate that the Anaphase Promoting Complex subunit 11 (APC11) gene carries a constitutive single-nucleotide exon. PMID: 26657562
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
RING-box family
Database Links

KEGG: ath:AT3G05870

STRING: 3702.AT3G05870.1

UniGene: At.26276

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