Recombinant Arabidopsis thaliana Annexin D1 (ANN1)

Code CSB-YP883003DOA
MSDS
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Source Yeast
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Code CSB-EP883003DOA
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Source E.coli
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Code CSB-EP883003DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP883003DOA
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Source Baculovirus
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Code CSB-MP883003DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ANN1
Uniprot No.
Alternative Names
ANN1; ANNAT1; ANX23-ATH; ATOXY5; OXY5; At1g35720; F14D7.2Annexin D1; AnnAt1; Annexin A1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-317
Target Protein Sequence
MATLKVSDSV PAPSDDAEQL RTAFEGWGTN EDLIISILAH RSAEQRKVIR QAYHETYGED LLKTLDKELS NDFERAILLW TLEPGERDAL LANEATKRWT SSNQVLMEVA CTRTSTQLLH ARQAYHARYK KSLEEDVAHH TTGDFRKLLV SLVTSYRYEG DEVNMTLAKQ EAKLVHEKIK DKHYNDEDVI RILSTRSKAQ INATFNRYQD DHGEEILKSL EEGDDDDKFL ALLRSTIQCL TRPELYFVDV LRSAINKTGT DEGALTRIVT TRAEIDLKVI GEEYQRRNSI PLEKAITKDT RGDYEKMLVA LLGEDDA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has a peroxidase activity. May act in counteracting oxidative stress. May also mediate regulated, targeted secretion of Golgi-derived vesicles during seedling development.
Gene References into Functions
  1. Results suggest that AtANN1 is important in regulating the heat-induced increase in [Ca(2+)]cytoplasmic and in the response of Arabidopsis seedlings to heat stress. PMID: 25587034
  2. AtANN1 is involved in shaping the root peroxide-induced cytosolic free Ca(2+) ([Ca(2+) ]cyt ) signature and downstream signalling. PMID: 24180429
  3. AtANN1 as a key component of root cell adaptation to salinity. [AtANN1] PMID: 23886625
  4. The Arabidopsis loss-of-function mutant for annexin1 (Atann1) was found to lack the root hair and epidermal hydroxyl radical-activated Ca(2)+ - and K+ -permeable conductance.[Annexin1] PMID: 22523205
  5. recombinant annexin 1 has peroxidase activity. PMID: 16153598
  6. The pH-sensitive ion channel activity of AnnAt1 may play a role in intracellular ion homeostasis. PMID: 17452342
  7. AnnAt1 has a role in oxidative protection. PMID: 19482919

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Subcellular Location
Cytoplasm, cytosol. Membrane. Note=translocate from cytosol to membrane upon salt treatment; this translocation is calcium dependent.
Protein Families
Annexin (TC 1.A.31.1) family
Tissue Specificity
Ubiquitous. Most abundant in stems.
Database Links

KEGG: ath:AT1G35720

STRING: 3702.AT1G35720.1

UniGene: At.25241

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