Recombinant Arabidopsis thaliana Arogenate dehydratase/prephenate dehydratase 2, chloroplastic (ADT2)

Code CSB-YP874505DOA
MSDS
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Source Yeast
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Code CSB-EP874505DOA
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Source E.coli
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Code CSB-EP874505DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP874505DOA
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Source Baculovirus
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Code CSB-MP874505DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ADT2
Uniprot No.
Alternative Names
ADT2; PDT2; At3g07630; MLP3.8; Arogenate dehydratase/prephenate dehydratase 2; chloroplastic; AtADT2; AtPDT2; EC 4.2.1.51; EC 4.2.1.91
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
67-381
Target Protein Sequence
AMEV KKIFEDSPLL PKPLSSNQLT ESVSNGSRVR VAYQGVRGAY SESAAEKAYP NCEAVPCEEF DTAFEAVERW LVDRAVLPIE NSLGGSIHRN YDLLLRHNLH IVGEVKLAVR HCLLANHGVN IEDLRRVLSH PQALAQCENT LTKLGLVREA VDDTAGAAKQ IAFENLNDAA AVASEKAAKI YGLNIVAKDI QDDCDNVTRF LMLAREPIIP GTNRLFKTSI VFSLEEGPGV LFKALAVFAL RQINLTKIES RPLRKHPLRA SGGLKYFDYL FYVDFEASMA DEVAQNALRH LEEFATFLRV LGSYPVDTTM L
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine.
Gene References into Functions
  1. ADT2 can use prephenate as a substrate and can function as a prephenate dehydratase PMID: 21388819
  2. Data show that rlaxed feedback inhibition and increased expression of the ADT2 mutant enzyme caused up to 160-fold higher accumulation of free phenylalanine in rosette leaves, as well as altered accumulation of several other metabolites. PMID: 20598094
Subcellular Location
Plastid, chloroplast stroma.
Tissue Specificity
Expressed in roots, leaves, stems, flowers and siliques. Most abundant in leaves and seeds.
Database Links

KEGG: ath:AT3G07630

STRING: 3702.AT3G07630.1

UniGene: At.22712

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