Recombinant Arabidopsis thaliana Aspartokinase 3, chloroplastic (AK3)

Code CSB-YP865812DOA
MSDS
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Source Yeast
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Code CSB-EP865812DOA
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Source E.coli
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Code CSB-EP865812DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865812DOA
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Source Baculovirus
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Code CSB-MP865812DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AK3
Uniprot No.
Alternative Names
AK3; AK-LYS3; At3g02020; F1C9.20; F28J7.35Aspartokinase 3; chloroplastic; EC 2.7.2.4; Aspartate kinase 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
86-559
Target Protein Sequence
VMKFG GSSVASAERM IQVAKLILSF PDEKPVVVLS AMAKTTNKLL MAGEKAVCCG VTNVDTIEEL SYIKELHIRT AHELGVETAV IAEHLEGLEQ LLKGVAMMKE LTLRSRDYLV SFGECMSTRL FAAYLNKIGH KARQYDAFEI GIITTDDFTN ADILEATYPA VSKKLLGDWS KENALPVVTG FLGKGWRSCA VTTLGRGGSD LTATTIGKAL GLREIQVWKD VDGVLTCDPN IYCGAQPVPH LTFDEAAELA YFGAQVLHPL SMRPAREGNI PVRVKNSYNP TAPGTVITRS RDMSKAVLTS IVLKRNVTML DITSTRMLGQ YGFLAKVFST FEKLGISVDV VATSEVSISL TLDPSKFCSR ELIQHELDQV VEELEKIAVV NLLRHRSIIS LIGNVQRSSF ILEKGFRVLR TNGINVQMIS QGASKVNISL IVNDDEAEHC VKALHSAFFE TDTCEAVSEC PTGYIAASS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the first step of essential amino acids lysine, threonine, methionine and isoleucine synthesis via the aspartate-family pathway.
Gene References into Functions
  1. Data show that mutations in the Asp kinases (AKs) and dual-functional Asp kinase-homoserine dehydrogenases (AK-HSDHs) result in reduced transcript levels and gene product activitiee and increases in the contents of Asp-derived amino acids. PMID: 26063505
  2. The Arabidopsis genome contains three genes for monofunctional aspartate kinases. AK2 and AK3 are inhibited only by lysine, and AK1 is inhibited in a synergistic manner by lysine and SAM. [AK3] [Aspartate kinase3] PMID: 17140415
Subcellular Location
Plastid, chloroplast.
Protein Families
Aspartokinase family
Tissue Specificity
Highly expressed in xylem of leaves and hypocotyls, stele of roots and in trichomes after bolting. Weak expression in veins and mesophyll cells of caulone leaves, inflorescence stems, sepals, petals and stigmata.
Database Links

KEGG: ath:AT3G02020

STRING: 3702.AT3G02020.1

UniGene: At.41192

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