Recombinant Arabidopsis thaliana Auxin-responsive protein IAA1 (IAA1)

Code CSB-YP341835DOA
MSDS
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Source Yeast
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Code CSB-EP341835DOA
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Source E.coli
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Code CSB-EP341835DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341835DOA
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Source Baculovirus
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Code CSB-MP341835DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IAA1
Uniprot No.
Alternative Names
IAA1; At4g14560; dl3320w; FCAALL.409; Auxin-responsive protein IAA1; Indoleacetic acid-induced protein 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-168
Target Protein Sequence
MEVTNGLNLK DTELRLGLPG AQEEQQLELS CVRSNNKRKN NDSTEESAPP PAKTQIVGWP PVRSNRKNNN NKNVSYVKVS MDGAPYLRKI DLKMYKNYPE LLKALENMFK FTVGEYSERE GYKGSGFVPT YEDKDGDWML VGDVPWDMFS SSCQKLRIMK GSEAPTAL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations. Repression is thought to result from the interaction with auxin response factors (ARFs), proteins that bind to the auxin-responsive promoter element (AuxRE). Formation of heterodimers with ARF proteins may alter their ability to modulate early auxin response genes expression.
Gene References into Functions
  1. Lysine-less IAA1 was ubiquitinated and rapidly degraded in vivo. PMID: 25888615
  2. The molecular cloning of AXR5 revealed that the gene encodes the IAA1 protein, a member of the Aux/IAA family of proteins. PMID: 15546359
  3. IAA1 might be involved in cell elongation as well as in cell division in the aerial parts of Arabidopsis plants. PMID: 18771815
  4. Iaa1-regulated auxin-response genes are controlled by ARFs to different extents. PMID: 19654206
Subcellular Location
Nucleus.
Protein Families
Aux/IAA family
Tissue Specificity
Preferentially expressed in stems, leaves and flowers.
Database Links

KEGG: ath:AT4G14560

STRING: 3702.AT4G14560.1

UniGene: At.43941

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