Recombinant Arabidopsis thaliana Bifunctional purple acid phosphatase 26 (PAP26)

Code CSB-YP839107DOA
MSDS
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Source Yeast
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Code CSB-EP839107DOA
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Source E.coli
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Code CSB-EP839107DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839107DOA
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Source Baculovirus
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Code CSB-MP839107DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PAP26
Uniprot No.
Alternative Names
PAP26; At5g34850; T5E15.10; Bifunctional purple acid phosphatase 26 [Includes: Acid phosphatase; EC 3.1.3.2); Peroxidase; EC 1.11.1.7)]
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
31-475
Target Protein Sequence
SEWPAVDIPL DHHVFKVPKG YNAPQQVHIT QGDYDGKAVI ISWVTPDEPG SSQVHYGAVQ GKYEFVAQGT YHNYTFYKYK SGFIHHCLVS DLEHDTKYYY KIESGESSRE FWFVTPPHVH PDASYKFGII GDMGQTFNSL STLEHYMESG AQAVLFLGDL SYADRYQYND VGVRWDSWGR FVERSTAYQP WLWSAGNHEV DYMPYMGEVT PFRNYLQRYT TPYLASKSSS PLWYAVRRAS AHIIVLSSYS PFVKYTPQWH WLSEELTRVD REKTPWLIVL MHVPIYNSNE AHFMEGESMR AAFEEWFVQH KVDVIFAGHV HAYERSYRIS NVRYNVSSGD RYPVPDKSAP VYITVGDGGN QEGLAGRFTE PQPDYSAFRE ASYGHSTLDI KNRTHAIYHW NRNDDGKKVA TDEFVLHNQY WGKNIRRRKL KKHYIRSVVG GWIAT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Metallo-phosphoesterase involved in phosphate metabolism. Acid phosphatase activity with phosphoenolpyruvate, inorganic pyrophosphate, phenyl-phosphate and p-nitrophenyl-phosphate as the most effective substrates. No activity with phytic acid, phosphocholine or bis-p-nitrophenyl-phosphate. Has a peroxidase activity at alkaline pH.
Gene References into Functions
  1. PAP26 has an important phosphorus remobilization function during leaf senescence. PMID: 23072540
  2. Enhanced synthesis of AtPAP26 during Pi stress is under post-transcriptional control. AtPAP12 and AtPAP26 facilitate Pi scavenging from soil-localized organophosphates during nutritional Pi deprivation. PMID: 20545876
  3. AtPAP26 is a principal contributor to phosphate (Pi) stress-inducible APase activity, and that it plays an important role in the Pi metabolism of -Pi Arabidopsis. PMID: 20348213
  4. The purification and characterization of PAP26 is reported. PMID: 16963519
Subcellular Location
Vacuole.
Protein Families
Metallophosphoesterase superfamily, Purple acid phosphatase family
Tissue Specificity
Expressed in roots, stems, leaves, flowers and siliques.
Database Links

KEGG: ath:AT5G34850

STRING: 3702.AT5G34850.1

UniGene: At.20088

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