Recombinant Arabidopsis thaliana Boron transporter 1 (BOR1), partial

Code CSB-YP837778DOA
MSDS
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Source Yeast
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Code CSB-EP837778DOA
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Source E.coli
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Code CSB-EP837778DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP837778DOA
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Source Baculovirus
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Code CSB-MP837778DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BOR1
Uniprot No.
Alternative Names
BOR1; At2g47160; T3D7.3Boron transporter 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Efflux-type boron (B) transporter for xylem loading, responsive of boron translocation from roots to shoots under boron limitation. Boron is essential for maintaining the integrity of plants cell walls.
Gene References into Functions
  1. Here, we present the 4.1-A resolution crystal structure of Arabidopsis thaliana Bor1. The structure displays a dimeric architecture in which dimerization is mediated by centralized Gate domains. Comparisons with a structure of Band 3 in an outward-open state reveal that the Core domains of Bor1 have rotated inwards to achieve an occluded state. PMID: 27601653
  2. Polar localization of BOR1 is dependent on DRP1-mediated endocytosis. PMID: 27449211
  3. Mutational analysis of AtBOR1 revealed that the acidic di-leucine motif is required for the polarity and high-Boron-induced vacuolar sorting of AtBOR1. PMID: 25619824
  4. In this chapter, we describe methods for observation of endocytic trafficking of BOR1, and detection of BOR1 ubiquitination that is required for vacuolar sorting for degradation PMID: 25117286
  5. The coat protein of Alternanthera mosaic virus interacts with host boron transporter 1. PMID: 24606704
  6. shown that both the polar localization and the endocytic degradation of BOR1 are controlled by at least two tyrosine residues in a large loop located in the cytosol PMID: 22301967
  7. boron-induced ubiquitination of BOR1 is not required for endocytosis from the PM but is crucial for the sorting of internalized BOR1 to multivesicular bodies for subsequent degradation in vacuoles. PMID: 21148314
  8. The present study of BOR1 and NIP5;1 demonstrates the importance of selective endocytic trafficking in polar localization and degradation of plant nutrient transporters for radial transport and homeostasis of plant mineral nutrients. PMID: 20194745
  9. The generation of Arabidopsis thaliana plants that tolerate boron (B)-deficient conditions due to the overexpression of BOR1 is reported. PMID: 16805739

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein.
Protein Families
Anion exchanger (TC 2.A.31.3) family
Tissue Specificity
Expressed in proximal side of various root cells, notably in the columella, lateral root cap, epidermis and endodermis in tip and elongation zones of the root. Also detected in the epidermis, cortex, endodermis, and stele cells of the root hair zone. Obse
Database Links

KEGG: ath:AT2G47160

STRING: 3702.AT2G47160.2

UniGene: At.24088

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