Recombinant Arabidopsis thaliana Branched-chain-amino-acid aminotransferase-like protein 3, chloroplastic (At5g57850)

Code CSB-YP811851DOA
MSDS
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Source Yeast
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Code CSB-EP811851DOA
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Source E.coli
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Code CSB-EP811851DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP811851DOA
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Source Baculovirus
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Code CSB-MP811851DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DAAT
Uniprot No.
Alternative Names
DAAT; BCAL3; At5g57850; MTI20.10D-amino-acid transaminase; chloroplastic; EC 2.6.1.21; EC 4.1.3.38; Aminodeoxychorismate lyase; AtADCL; Branched-chain-amino-acid aminotransferase-like protein 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
58-373
Target Protein Sequence
SSY EVGERLKLAR GGQQFLAMYS SVVDGITTDP AAMVLPLDDH MVHRGHGVFD TALIINGYLY ELDQHLDRIL RSASMAKIPL PFDRETIKRI LIQTVSVSGC RDGSLRYWLS AGPGDFLLSP SQCLKPTLYA IVIKTNFAIN PIGVKVVTSS IPIKPPEFAT VKSVNYLPNV LSQMEAEAKG AYAGIWVCKD GFIAEGPNMN VAFVVNGGKE LVMPRFDNVL SGCTAKRTLT LAEQLVSKGI LKTVKVMDVT VEDGKKADEM MLIGSGIPIR PVIQWDEEFI GEGKEGPIAK ALLDLLLEDM RSGPPSVRVL VPY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Amino acid aminotransferase showing activity for D-Asp and D-Ala as amino donors with 2-oxoglutarate as an amino acceptor. Can also use D-Met, D-Tyr, D-Phe, D-Gln, D-Trp and D-Asn as substrates, but no activity with L-Asp, L-Ala, L-Leu, L-Ile or L-Val. Catalyzes also the reverse reaction where an amino group is transferred from D-Glu to pyruvate or oxaloacetate to produce D-Ala or D-Asp, respectively. Also involved in folate biosynthesis, acting as an aminodeoxychorismate lyase converting 4-amino-4-deoxychorismate (ADC) to p-aminobenzoate (PABA)(PubMed:15500462).
Gene References into Functions
  1. The full-length Arabidopsis ADC lyase polypeptide was translocated into isolated pea chloroplasts and directed the passenger protein to Arabidopsis chloroplasts. [ADC lyase] PMID: 15500462
Subcellular Location
Plastid, chloroplast.
Protein Families
Class-IV pyridoxal-phosphate-dependent aminotransferase family
Database Links

KEGG: ath:AT5G57850

STRING: 3702.AT5G57850.1

UniGene: At.7653

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