Recombinant Arabidopsis thaliana C-terminal binding protein AN (AN), partial

Code CSB-YP517815DOA
MSDS
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Source Yeast
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Code CSB-EP517815DOA
MSDS
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Source E.coli
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Code CSB-EP517815DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP517815DOA
MSDS
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Source Baculovirus
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Code CSB-MP517815DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AN
Uniprot No.
Alternative Names
AN; DOQ; At1g01510; F22L4.6C-terminal binding protein AN; CtBP; Protein ANGUSTIFOLIA; Protein DETORQUEO
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Required for cortical microtubules (MTs) arrangement that confers cell shape. Regulates the width of leaves by controlling the polar elongation of leaf cells. Involved in the regulation of trichome branching. Seems to not be able to regulate gene transcription. Regulates epidermal cell divisions and elongation in a non-cell-autonomous manner (regulated by subepidermal cells), but regulates epidermal cell polarity, shape, trichome branching and elongation in a cell-autonomous manner. Negatively regulates growth in the petiole elongation. Prevents lipid peroxidation as a result of abiotic stress response. Is involved in the SUB-dependent signaling mechanism and may act in a membrane trafficking event around the trans-Golgi network.
Gene References into Functions
  1. ANGUSTIFOLIA participates in controlling cell morphogenesis, abiotic, and biotic stress responses. PMID: 23672620
  2. Data show that subepidermal rescue of leaf width is accompanied by increased cell number in the epidermis, indicating that AN can trigger cell divisions in a non-autonomous manner. PMID: 19843316
Subcellular Location
Cytoplasm. Golgi apparatus, trans-Golgi network.
Protein Families
D-isomer specific 2-hydroxyacid dehydrogenase family, Plant AN subfamily
Tissue Specificity
Expressed in cotyledons, leaves, roots, stems and floral buds.
Database Links

KEGG: ath:AT1G01510

STRING: 3702.AT1G01510.1

UniGene: At.214

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