Recombinant Arabidopsis thaliana COP9 signalosome complex subunit 5a (CSN5A)

Code CSB-YP875544DOA
MSDS
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Source Yeast
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Code CSB-EP875544DOA
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Source E.coli
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Code CSB-EP875544DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875544DOA
MSDS
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Source Baculovirus
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Code CSB-MP875544DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CSN5B
Uniprot No.
Alternative Names
CSN5B; AJH2; CSN5A; At1g71230; F3I17.12COP9 signalosome complex subunit 5b; Signalosome subunit 5b; EC 3.4.-.-; Jun activation domain-binding homolog 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-358
Target Protein Sequence
MEGSSSTIAR KTWELENSIL TVDSPDSTSD NIFYYDDTSQ TRFQQEKPWE NDPHYFKRVK ISALALLKMV VHARSGGTIE IMGLMQGKTD GDTIIVMDAF ALPVEGTETR VNAQDDAYEY MVEYSQTNKL AGRLENVVGW YHSHPGYGCW LSGIDVSTQR LNQQHQEPFL AVVIDPTRTV SAGKVEIGAF RTYSKGYKPP DEPVSEYQTI PLNKIEDFGV HCKQYYSLDV TYFKSSLDSH LLDLLWNKYW VNTLSSSPLL GNGDYVAGQI SDLAEKLEQA ESHLVQSRFG GVVPSSLHKK KEDESQLTKI TRDSAKITVE QVHGLMSQVI KDELFNSMRQ SNNKSPTDSS DPDPMITY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. The CSN complex is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of PSIAA6 by regulating the activity of the Ubl ligase SCF-TIR complex. Not involved in CSN's deneddylation/derubylation activity. Essential for the structural integrity of the CSN holocomplex.
Gene References into Functions
  1. a regulatory role of CSN5B in light-dark regulation of ascorbic acid synthesis PMID: 23424245
  2. not essential for auxin responses PMID: 18467458
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
Peptidase M67A family, CSN5 subfamily
Tissue Specificity
Ubiquitously expressed. Highly expressed in flowers and roots. Expressed at lower level in seedlings and siliques.
Database Links

KEGG: ath:AT1G71230

STRING: 3702.AT1G71230.1

UniGene: At.17922

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