Recombinant Arabidopsis thaliana Casein kinase II subunit beta (CKB1)

Code CSB-YP006075DOA
MSDS
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Source Yeast
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Code CSB-EP006075DOA
MSDS
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Source E.coli
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Code CSB-EP006075DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006075DOA
MSDS
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Source Baculovirus
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Code CSB-MP006075DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CKB1
Uniprot No.
Alternative Names
CKB1; At5g47080; K14A3.3Casein kinase II subunit beta-1; CK II beta-1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-287
Target Protein Sequence
MYRDRGTVNS RPEVVDRKRI NDALERPSPS TSRQVNGKGK GTVTAATTTA NLIGKQQSNN INHRDSRSAS LSKNNTVSDD ESDTDSEESD VSGSDGEDTS WISWFCNLRG NEFFCEVDDD YIQDDFNLCG LSSLVPYYEY ALDLILDVES SQGEMFTEEQ NELIESAAEM LYGLIHARYI LTSKGLAAML DKYKNYDFGR CPRVYCCGQP CLPVGQSDLP RSSTVKIYCP KCEDIYYPRS KYQGNIDGAY FGTTFPHLFL MTYGHLKPAK ATQNYVQRVF GFKLHKP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a complex role in regulating the basal catalytic activity of the alpha subunit. The tetrameric holoenzyme CK2, composed of two alpha and two beta subunits, phosphorylates the transcription factor GBFl, resulting in stimulation of its DNA binding activity. CK2 phosphorylates the transcription factor PIF1 after an exposure to light, resulting in a proteasome-dependent degradation of PIF1 and promotion of photomorphogenesis. CK2 phosphorylates translation initiation factors. May participate in the regulation of the initiation of translation. Stimulates the binding of CCA1 to promoters (Probable).
Subcellular Location
Cytoplasm, cytosol. Nucleus.
Protein Families
Casein kinase 2 subunit beta family
Database Links

KEGG: ath:AT5G47080

STRING: 3702.AT5G47080.1

UniGene: At.23955

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