Recombinant Arabidopsis thaliana Catalase-2 (CAT2)

Code CSB-YP333109DOA
MSDS
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Source Yeast
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Code CSB-EP333109DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333109DOA
MSDS
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Source Baculovirus
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Code CSB-MP333109DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CAT2
Uniprot No.
Alternative Names
CAT2; CAT; At4g35090; M4E13.140Catalase-2; EC 1.11.1.6
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-492
Target Protein Sequence
MDPYKYRPAS SYNSPFFTTN SGAPVWNNNS SMTVGPRGPI LLEDYHLVEK LANFDRERIP ERVVHARGAS AKGFFEVTHD ISNLTCADFL RAPGVQTPVI VRFSTVIHER GSPETLRDPR GFAVKFYTRE GNFDLVGNNF PVFFIRDGMK FPDMVHALKP NPKSHIQENW RILDFFSHHP ESLNMFTFLF DDIGIPQDYR HMDGSGVNTY MLINKAGKAH YVKFHWKPTC GVKSLLEEDA IRVGGTNHSH ATQDLYDSIA AGNYPEWKLF IQIIDPADED KFDFDPLDVT KTWPEDILPL QPVGRMVLNK NIDNFFAENE QLAFCPAIIV PGIHYSDDKL LQTRVFSYAD TQRHRLGPNY LQLPVNAPKC AHHNNHHEGF MNFMHRDEEV NYFPSRYDQV RHAEKYPTPP AVCSGKRERC IIEKENNFKE PGERYRTFTP ERQERFIQRW IDALSDPRIT HEIRSIWISY WSQADKSLGQ KLASRLNVRP SI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
Gene References into Functions
  1. The cat1/2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditions compared with wild-type and the cat2/3 double mutants. PMID: 29575603
  2. CAT2 functions in early seedling growth by scavenging hydrogen peroxide and stimulating ACX2/3 activity. PMID: 28722222
  3. SHORT-ROOT Deficiency Alleviates the Cell Death Phenotype of the Arabidopsis catalase2 Mutant under Photorespiration-Promoting Conditions PMID: 27432873
  4. CAT2 role in photorespiration. PMID: 27225899
  5. CAT2 coordinates salicylic acid repression of auxin accumulation and jasmonic acid biosynthesis in plant defense PMID: 28182949
  6. The CAT2 promoter is regulated by the ACGT motif (Box2) rather than by the G-Box binding motif (Box1) in the vegetative phase of development. PMID: 27734290
  7. CAT2 and NCA1 promote autophagy-dependent cell death in Arabidopsis. PMID: 24285797
  8. The hyponastic leaves of cat2-1 reveal crosstalk between H2 O2 and auxin signalling that is mediated by changes in glutathione redox status. PMID: 23738953
  9. NADP-ME2 transcripts accumulated in response to ozone or in mutants undergoing intracellular oxidative stress. Loss of NADP-ME2 function had little effect on cat2-triggered changes in glutathione or NADPH. PMID: 23851193
  10. Introducing the cad2 mutation into cat2 shows that the cad2 mutation overrode the main features of the cat2 effect on thiol profiles. PMID: 23210597
  11. Data indicate that in three different configurations, primary silencing signals were able to direct in trans methylation of chimeric transgenes and the CATALASE2 (CAT2) endogene. PMID: 23480471
  12. the roles of catalase (CAT) in abscisic acid (ABA)-induced stomatal closure PMID: 21979081
  13. Studies indicate that in apx1/cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint. PMID: 21220338
  14. Using CAT2 as an example, it was demonstrated that increasing the distance between direct and adjacent target sequences in a transgenic primary target delays the onset of silencing of a secondary target gene PMID: 16891552
  15. CAT2 expression is down-regulated during leaf senescence PMID: 17080932
  16. Data suggest that the interaction of SOS2 with both NDPK2 and CATs2 and 3 reveals a point of cross talk between salt stress response and other signaling factors including H2O2. PMID: 17785451
  17. Data show that cat2-1 mutant phenotype include more H(2)O(2), reduced size, paler green color, smaller secondary roots and increased sensitivity to H(2)O(2), NaCl, norspermidine, high light and cold stress. The germination is more tolerant to lithium. PMID: 17931347

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Subcellular Location
Cytoplasm. Peroxisome. Glyoxysome.
Protein Families
Catalase family
Database Links

KEGG: ath:AT4G35090

STRING: 3702.AT4G35090.1

UniGene: At.24350

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