Recombinant Arabidopsis thaliana Catalase-2 (CAT2)

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Code CSB-EP333109DOA
MSDS
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
CAT2
Uniprot No.
Research Area
Others
Alternative Names
CAT2; CAT; At4g35090; M4E13.140Catalase-2; EC 1.11.1.6
Species
Arabidopsis thaliana (Mouse-ear cress)
Source
E.coli
Expression Region
1-492aa
Target Protein Sequence
MDPYKYRPASSYNSPFFTTNSGAPVWNNNSSMTVGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFIRDGMKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLNMFTFLFDDIGIPQDYRHMDGSGVNTYMLINKAGKAHYVKFHWKPTCGVKSLLEEDAIRVGGTNHSHATQDLYDSIAAGNYPEWKLFIQIIDPADEDKFDFDPLDVTKTWPEDILPLQPVGRMVLNKNIDNFFAENEQLAFCPAIIVPGIHYSDDKLLQTRVFSYADTQRHRLGPNYLQLPVNAPKCAHHNNHHEGFMNFMHRDEEVNYFPSRYDQVRHAEKYPTPPAVCSGKRERCIIEKENNFKEPGERYRTFTPERQERFIQRWIDALSDPRITHEIRSIWISYWSQADKSLGQKLASRLNVRPSI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
72.9kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
Gene References into Functions
  1. The cat1/2/3 triple mutants displayed severe redox disturbance and growth defects under physiological conditions compared with wild-type and the cat2/3 double mutants. PMID: 29575603
  2. CAT2 functions in early seedling growth by scavenging hydrogen peroxide and stimulating ACX2/3 activity. PMID: 28722222
  3. SHORT-ROOT Deficiency Alleviates the Cell Death Phenotype of the Arabidopsis catalase2 Mutant under Photorespiration-Promoting Conditions PMID: 27432873
  4. CAT2 role in photorespiration. PMID: 27225899
  5. CAT2 coordinates salicylic acid repression of auxin accumulation and jasmonic acid biosynthesis in plant defense PMID: 28182949
  6. The CAT2 promoter is regulated by the ACGT motif (Box2) rather than by the G-Box binding motif (Box1) in the vegetative phase of development. PMID: 27734290
  7. CAT2 and NCA1 promote autophagy-dependent cell death in Arabidopsis. PMID: 24285797
  8. The hyponastic leaves of cat2-1 reveal crosstalk between H2 O2 and auxin signalling that is mediated by changes in glutathione redox status. PMID: 23738953
  9. NADP-ME2 transcripts accumulated in response to ozone or in mutants undergoing intracellular oxidative stress. Loss of NADP-ME2 function had little effect on cat2-triggered changes in glutathione or NADPH. PMID: 23851193
  10. Introducing the cad2 mutation into cat2 shows that the cad2 mutation overrode the main features of the cat2 effect on thiol profiles. PMID: 23210597
  11. Data indicate that in three different configurations, primary silencing signals were able to direct in trans methylation of chimeric transgenes and the CATALASE2 (CAT2) endogene. PMID: 23480471
  12. the roles of catalase (CAT) in abscisic acid (ABA)-induced stomatal closure PMID: 21979081
  13. Studies indicate that in apx1/cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint. PMID: 21220338
  14. Using CAT2 as an example, it was demonstrated that increasing the distance between direct and adjacent target sequences in a transgenic primary target delays the onset of silencing of a secondary target gene PMID: 16891552
  15. CAT2 expression is down-regulated during leaf senescence PMID: 17080932
  16. Data suggest that the interaction of SOS2 with both NDPK2 and CATs2 and 3 reveals a point of cross talk between salt stress response and other signaling factors including H2O2. PMID: 17785451
  17. Data show that cat2-1 mutant phenotype include more H(2)O(2), reduced size, paler green color, smaller secondary roots and increased sensitivity to H(2)O(2), NaCl, norspermidine, high light and cold stress. The germination is more tolerant to lithium. PMID: 17931347

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Subcellular Location
Cytoplasm. Peroxisome. Glyoxysome.
Protein Families
Catalase family
Database Links

KEGG: ath:AT4G35090

STRING: 3702.AT4G35090.1

UniGene: At.24350

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