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Recombinant Arabidopsis thaliana Cation/H (+) antiporter 21 (CHX21), partial

Recombinant Arabidopsis thaliana Cation/H (+) antiporter 21 (CHX21), partial

Code	CSB-YP890371DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP890371DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP890371DOA-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP890371DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP890371DOA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

CHX21

Uniprot No.

Q9SKA9

Alternative Names

CHX21; At2g31910; F20M17.5; Cation/H(+) antiporter 21; Protein CATION/H+ EXCHANGER 21; AtCHX21

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Operates as a Na(+)/H(+) antiporter that plays a role in regulation of xylem Na(+) concentration and, consequently, Na(+) accumulation in the leaf. Required for pollen tube guidance, but not for normal pollen development. May also be involved in the development or function of the female gametophyte.

Gene References into Functions

conclusion is that both AtCHX21 and AtCHX23 act in potassium homeostasis within the female gametophyte and this is discussed in terms of the diversification of gene sequence and function within the CHX gene family PMID: 21976771
CHX21 and CHX23, participate in pollen tube guidance and are functionally redundant. PMID: 21239645
The CHX21 transporter plays a role in the regulation of xylem Na+ concentration and, consequently, Na+ accumulation in the leaf. PMID: 16513816

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family, CHX (TC 2.A.37.4) subfamily

Tissue Specificity

Specifically expressed in root endodermal cells. Expressed in seedlings, roots, leaves, flowers, flower buds and pollen.

Database Links

KEGG: ath:AT2G31910

STRING: 3702.AT2G31910.1

UniGene: At.38150

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