Recombinant Arabidopsis thaliana Cation/H (+) antiporter 23, chloroplastic (CHX23), partial

Code CSB-YP851887DOA
MSDS
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Source Yeast
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Code CSB-EP851887DOA
MSDS
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Source E.coli
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Code CSB-EP851887DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP851887DOA
MSDS
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Source Baculovirus
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Code CSB-MP851887DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CHX23
Uniprot No.
Alternative Names
CHX23; At1g05580; F3F20.2Cation/H(+) antiporter 23; chloroplastic; Protein CATION/H+ EXCHANGER 23; AtCHX23
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Operates as a K(+)/H(+) antiporter or Na(+)/H(+) antiporter of the chloroplast envelope that functions in pH homeostasis and chloroplast development. Monovalent cation transporter with a preference for Cs(+), K(+) and Rb(+) relative to Na(+) or Li(+). Required for pollen tube guidance, but not for normal pollen development. May also be involved in the development or function of the female gametophyte.
Subcellular Location
Plastid, chloroplast membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=PubMed:15220473 shows a localization in chloroplasts while PubMed:21239645 shows a localization in ER.
Protein Families
Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family, CHX (TC 2.A.37.4) subfamily
Tissue Specificity
Specifically expressed in flower buds and pollen. Expressed in leaves, roots and stems.
Database Links

KEGG: ath:AT1G05580

STRING: 3702.AT1G05580.1

UniGene: At.42390

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