Recombinant Arabidopsis thaliana Cellulose synthase A catalytic subunit 3 [UDP-forming] (CESA3), partial

Code CSB-YP853183DOA
MSDS
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Source Yeast
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Code CSB-EP853183DOA
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Source E.coli
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Code CSB-EP853183DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP853183DOA
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Source Baculovirus
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Code CSB-MP853183DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CESA3
Uniprot No.
Alternative Names
CESA3; ATHB; CEV1; ELI1; IXR1; RSW5; At5g05170; K2A11.4Cellulose synthase A catalytic subunit 3 [UDP-forming]; AtCesA3; EC 2.4.1.12; Constitutive expression of VSP1 protein 1; Isoxaben-resistant protein 1; Ath-B; Protein ECTOPIC LIGNIN 1; Protein RADIALLY SWOLLEN 5; AtRSW5
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation. Involved in the primary cell wall formation, especially in roots.
Gene References into Functions
  1. The phenotypes observed in the trans-heterozygotes indicate that the mre1 and cev1 alleles of the AtCesA3 gene display intragenic complementation. PMID: 26391708
  2. The cell walls in the roots of these four AtCESA3 alleles contain different percentages of cellulose, and these percentages correlate with the lengths of the roots and cortex cells in these roots when grown on media containing high levels of sucrose. PMID: 22514801
  3. Data test that hypothesis using Arabidopsis plants expressing the cellulose synthase GFP-CESA3 and show that cellulose synthase trajectories do rotate over several hours. PMID: 20876662
  4. The incorporation of a mis-folded CesA3 subunit into the cellulose synthase complex may stall or prevent the formation of functional rosette complexes. PMID: 19645738
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Glycosyltransferase 2 family, Plant cellulose synthase subfamily
Tissue Specificity
Expressed in young plants, flowers and roots, and to a lower extent in leaves and stems. Localized in all cells except meristematic cells. Accumulates particularly in root caps, root hairs, epidermal layer, midveins of leaves and anthers. Not present in o
Database Links

KEGG: ath:AT5G05170

STRING: 3702.AT5G05170.1

UniGene: At.24338

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