Recombinant Arabidopsis thaliana Chaperone protein dnaJ 3 (ATJ3)

Code CSB-YP846410DOA
MSDS
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Source Yeast
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Code CSB-EP846410DOA
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Source E.coli
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Code CSB-EP846410DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP846410DOA
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Source Baculovirus
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Code CSB-MP846410DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ATJ3
Uniprot No.
Alternative Names
ATJ3; A3; J3; At3g44110; F26G5.60Chaperone protein dnaJ 3; AtDjA3; AtJ3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-417
Target Protein Sequence
MFGRGPSKKS DNTKFYEILG VPKSASPEDL KKAYKKAAIK NHPDKGGDPE KFKELAQAYE VLSDPEKREI YDQYGEDALK EGMGGGGGGH DPFDIFSSFF GGGPFGGNTS RQRRQRRGED VVHPLKVSLE DVYLGTMKKL SLSRNALCSK CNGKGSKSGA SLKCGGCQGS GMKVSIRQLG PGMIQQMQHA CNECKGTGET INDRDRCPQC KGDKVIPEKK VLEVNVEKGM QHSQKITFEG QADEAPDTVT GDIVFVLQQK EHPKFKRKGE DLFVEHTLSL TEALCGFQFV LTHLDGRSLL IKSNPGEVVK PDSYKAISDE GMPIYQRPFM KGKLYIHFTV EFPDSLSPDQ TKALEAVLPK PSTAQLSDME IDECEETTLH DVNIEDEMRR KAQAQREAYD DDDEDDDHPG GAQRVQC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a continuous role in plant development probably in the structural organization of compartments.
Gene References into Functions
  1. defective farnesylation of a single factor-heat-shock protein 40 (HSP40), encoded by the J2 and J3 genes-is sufficient to confer abscisic acid hypersensitivity, drought resistance, late flowering, and enlarged meristems. PMID: 29269486
  2. Data suggest that J3 activity in leaves mainly contributes to its role in regulating flowering time PMID: 21494090
  3. Loss of function of J3 delays flowering time in Arabidopsis, and regulates the expression of SOC1 and FT. PMID: 21343416
  4. Results indicate that the expression of AtJ2 and AtJ3 genes is involved in response to many environmental stresses except salt stress. PMID: 15692178
Subcellular Location
Membrane; Lipid-anchor.
Protein Families
DnaJ family, A/I subfamily
Tissue Specificity
Roots, shoots, flowers, siliques and cotyledons.
Database Links

KEGG: ath:AT3G44110

STRING: 3702.AT3G44110.1

UniGene: At.20860

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