Recombinant Arabidopsis thaliana Chitinase-like protein 1 (CTL1)

Code CSB-YP863009DOA
MSDS
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Source Yeast
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Code CSB-EP863009DOA
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Source E.coli
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Code CSB-EP863009DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863009DOA
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Source Baculovirus
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Code CSB-MP863009DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CTL1
Uniprot No.
Alternative Names
CTL1; ARM; ELP1; ERH2; HOT2; POM1; At1g05850; T20M3.12Chitinase-like protein 1; AtCTL1; Protein ANION ALTERED ROOT MORPHOLOGY; Protein ECTOPIC DEPOSITION OF LIGNIN IN PITH 1; Protein ECTOPIC ROOT HAIR 2; Protein POM-POM1; Protein SENSITIVE TO HOT TEMPERATURES 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
27-321
Target Protein Sequence
EDKT IKVKKVRGNK VCTQGWECSW WSKYCCNQTI SDYFQVYQFE QLFSKRNTPI AHAVGFWDYQ SFITAAALFE PLGFGTTGGK LMGQKEMAAF LGHVASKTSC GYGVATGGPL AWGLCYNREM SPMQSYCDES WKFKYPCSPG AEYYGRGALP IYWNFNYGAA GEALKADLLN HPEYIEQNAT LAFQAAIWRW MTPIKRAQPS AHDIFVGNWK PTKNDTLSKR GPTFGSTMNV LYGEYTCGQG SIDPMNNIIS HYLYFLDLMG IGREDAGPND ELSCAEQKPF NPSTVPSSSS S
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
No chitinase activity. Essential for normal plant growth and development. Regulates cell expansion extent and differentiation at least in roots and hypocotyls. Prevents lignin accumulation in the pith. May modulate ethylene-mediated regulation during development. Probably required to establish thermotolerance acclimation. Plays a role for controlled anisotropic cell expansion in the regulation of waving during root gravitropism and thigmotropism. Involved in the root system architecture adaptation to multiple environmental conditions such as nitrate. Contributes to salt tolerance and possibly to drought by preventing the overaccumulation of sodium ions.
Gene References into Functions
  1. Data indicate a role of the CHITINASE-LIKE 1 (CTL1) gene, a giant cells (GCs) repressed gene. PMID: 29389847
  2. Consistent with its function in the elaboration of the sieve areas, AtCTL1 has a sustained, polar localization in the forming sieve plates. PMID: 25008948
  3. CTL1 affects primary wall cellulose deposition. CTL1 and CTL2 influence the cellulose assembly and are involved in cross-linking of glucan-based polysaccharides. PMID: 22327741
  4. The spatial pattern of CTL1 expression was mainly in the root and root tips during seedling development and that the protein localized to the cell wall. PMID: 21949212
  5. AtCTL1 plays a role in plant growth and development wherein has a constitutive effect on cell wall synthesis and cell elongation, and its function is modulated by environmental stimuli, such as high nitrate concentration, that alter organ morphology. PMID: 20007445

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Subcellular Location
Secreted.
Protein Families
Glycosyl hydrolase 19 family
Tissue Specificity
Mostly expressed in seedlings shoots and roots, stems, and flowers, and, to a lower extent, in flowers, mature leaves and roots.
Database Links

KEGG: ath:AT1G05850

STRING: 3702.AT1G05850.1

UniGene: At.24593

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