Recombinant Arabidopsis thaliana Chlorophyllide a oxygenase, chloroplastic (CAO)

Code CSB-YP863017DOA
MSDS
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Source Yeast
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Code CSB-EP863017DOA
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Source E.coli
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Code CSB-EP863017DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863017DOA
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Source Baculovirus
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Code CSB-MP863017DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CAO
Uniprot No.
Alternative Names
CAO; CHL; At1g44446; T18F15.7; Chlorophyllide a oxygenase; chloroplastic; Chlorophyll a oxygenase; EC 1.14.13.122; Chlorophyll b synthase; AtCAO
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
37-536
Target Protein Sequence
VFAV FGDESGLVEK KSQWRPLFDV EDPRSKAPPY KGKFLDVNQA IEVARFDIQY LDWRARQDLL TIMILHDKVV DVLNPLAREY KSIGTVKKEL AGLQEELSKA HQQVHISEAR VSTALDKLAH MEELVNDRLL PGRVVTELDK PSSSTTASAV ELDREKTNTG AKSLNVSGPV PPYSPHLKNF WYPVAFTADL KHDTMVPIEC FEQPWVIFRG EDGKPGCVRN TCAHRACPLD LGTVNEGRIQ CPYHGWEYST DGECKKMPST KLLKVKIKSL PCLEQEGMIW IWPGDEPPAP ILPSLQPPSG FLIHAELVMD LPVEHGLLLD NLLDLAHAPF THTSTFAKGW SVPSLVKFLT PTSGLQGYWD PYPIDMEFKP PCIVLSTIGI SKPGKLEGKS TQQCATHLHQ LHVCLPSSKN KTRLLYRMSL DFAPILKNLP FMEHLWRHFA EQVLNEDLRL VLGQQERMLN GANIWNLPVA YDKLGVRYRL WRNAVDRGDD KLPFSG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes a two-step oxygenase reaction involved in the synthesis of chlorophyll b. Acts specifically on the non-esterified chlorophyllide a and not on chlorophyll a.
Gene References into Functions
  1. Data indicate that jasmonate biosynthesis was strongly induced in CH1 (At1g44446) mutant plants under high-light stress. PMID: 23590883
  2. The mechanism which regulates Chl a/b ratios and whether the changes in Chl a/b ratios are essential in regulation of LHC accumulation during light acclimation was studied. PMID: 16170635
  3. CAO is involved in the regulated import and stabilization of the chlorophyllide b-binding light-harvesting proteins Lhcb1 (LHCII) and Lhcb4 (CP29) in chloroplasts. PMID: 16537436
  4. Data show that the A domain of CAO is essential in the control of chlorophyll biosynthesis and in the survival of seedlings during de-etiolation especially under strong illumination. PMID: 18549471
  5. Determination of a chloroplast degron in the regulatory domain of chlorophyllide a oxygenase. PMID: 19843523

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Subcellular Location
Plastid, chloroplast membrane; Peripheral membrane protein. Plastid, chloroplast thylakoid membrane; Peripheral membrane protein.
Database Links

KEGG: ath:AT1G44446

STRING: 3702.AT1G44446.1

UniGene: At.19047

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