Recombinant Arabidopsis thaliana Clp protease-related protein At4g12060, chloroplastic (At4g12060)

Code CSB-YP818110DOA
MSDS
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Source Yeast
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Code CSB-EP818110DOA
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Source E.coli
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Code CSB-EP818110DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818110DOA
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Source Baculovirus
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Code CSB-MP818110DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLPT2
Uniprot No.
Alternative Names
CLPT2; CLPS2; At4g12060; F16J13.130ATP-dependent Clp protease ATP-binding subunit CLPT2; chloroplastic
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
59-241
Target Protein Sequence
SA LQTNRPRRIH KSAISSLPTA NPDLVVSDAK KPKWSWRAIK SFAMGELEAR KLKYPNTGTE ALLMGILIEG TSFTSKFLRA NKIMLYKVRE ETVKLLGKAD MYFFSPEHPP LTEDAQRALD SALDQNLKAG GIGEVMPAHI LLGIWSEVES PGHKILATLG FTDEKSKELE SFASESGFLD E
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Accessory protein regulating the assembly of the plastidial Clp protease system. CLPT1 first binds to the heptameric P-ring containing the CLP3-6 subunits followed by CLPT2, and only then does the P-ring combine with the R-ring composed of the clpP1 and CLPR1-4 subunits. Once the core complex is fully assembled, it then associates to the CLPC chaperone partner to form the functional protease. CLPT2 and CLPT1 are partially redundant.
Gene References into Functions
  1. In vivo and in vitro assays showed that ClpT1 and ClpT2 can independently interact with the single ClpP ring and ClpPR core, but not with the single ClpR ring. PMID: 25921872
  2. ClpT1 and ClpT2 regulate the assembly of the Clp proteolytic core. PMID: 21266658
Subcellular Location
Plastid, chloroplast.
Protein Families
ClpA/ClpB family
Database Links

KEGG: ath:AT4G12060

STRING: 3702.AT4G12060.1

UniGene: At.43268

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