Recombinant Arabidopsis thaliana Cysteine desulfurase 2, chloroplastic (NFS2)

Code CSB-YP856671DOA
MSDS
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Source Yeast
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Code CSB-EP856671DOA
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Source E.coli
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Code CSB-EP856671DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856671DOA
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Source Baculovirus
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Code CSB-MP856671DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NFS2
Uniprot No.
Alternative Names
NFS2; CpNIFS; CpNIFS1; At1g08490; T27G7.17Cysteine desulfurase 1; chloroplastic; EC 2.8.1.7; NIFS-like protein 1; CpNifS1; Plastid sufS-like protein; Protein AtCpNifS; Selenocysteine lyase; EC 4.4.1.16
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
36-463
Target Protein Sequence
AAAAS SATISTDSES VSLGHRVRKD FRILHQEVNG SKLVYLDSAA TSQKPAAVLD ALQNYYEFYN SNVHRGIHYL SAKATDEFEL ARKKVARFIN ASDSREIVFT RNATEAINLV AYSWGLSNLK PGDEVILTVA EHHSCIVPWQ IVSQKTGAVL KFVTLNEDEV PDINKLRELI SPKTKLVAVH HVSNVLASSL PIEEIVVWAH DVGAKVLVDA CQSVPHMVVD VQKLNADFLV ASSHKMCGPT GIGFLYGKSD LLHSMPPFLG GGEMISDVFL DHSTYAEPPS RFEAGTPAIG EAIALGAAVD YLSGIGMPKI HEYEVEIGKY LYEKLSSLPD VRIYGPRPSE SVHRGALCSF NVEGLHPTDL ATFLDQQHGV AIRSGHHCAQ PLHRYLGVNA SARASLYFYN TKDDVDAFIV ALADTVSFFN SFK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine. Supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. Required for the maturation of all plastidic Fe-S proteins and, thus, essential for plant growth.
Gene References into Functions
  1. CpNifS was sufficient for Fe-S cluster formation in ferredoxin in the presence of cysteine and a ferrous iron salt. [CpNifS] PMID: 15480755
  2. overexpression of this plant NifS-like protein had a pronounced effect on plant Se metabolism PMID: 16244144
  3. CpNifS is necessary for the maturation of all plastidic Fe-S proteins and, thus, essential for plant growth PMID: 17372218
Subcellular Location
Plastid, chloroplast. Plastid, chloroplast stroma.
Protein Families
Class-V pyridoxal-phosphate-dependent aminotransferase family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT1G08490

STRING: 3702.AT1G08490.1

UniGene: At.22935

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