Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 2 (CRRSP2)

Code CSB-YP808407DOA
MSDS
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Source Yeast
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Code CSB-EP808407DOA
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Source E.coli
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Code CSB-EP808407DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP808407DOA
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Source Baculovirus
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Code CSB-MP808407DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CRRSP2
Uniprot No.
Alternative Names
PDLP5; CRRSP2; HWI1; At1g70690; F5A18.13; Plasmodesmata-located protein 5; PD-located protein 5; Cysteine-rich repeat protein HWI1; Cysteine-rich repeat secretory protein 2; Plasmodesmata localizing protein 5; Protein HOPW1-1-INDUCED 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
26-299
Target Protein Sequence
SPTDN YIYAVCSPAK FSPSSGYETN LNSLLSSFVT STAQTRYANF TVPTGKPEPT VTVYGIYQCR GDLDPTACST CVSSAVAQVG ALCSNSYSGF LQMENCLIRY DNKSFLGVQD KTLILNKCGQ PMEFNDQDAL TKASDVIGSL GTGDGSYRTG GNGNVQGVAQ CSGDLSTSQC QDCLSDAIGR LKSDCGMAQG GYVYLSKCYA RFSVGGSHAR QTPGPNFGHE GEKGNKDDNG VGKTLAIIIG IVTLIILLVV FLAFVGKCCR KLQDEKWCK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Modulates cell-to-cell trafficking. Has a positive role in innate immunity. Required for systemic acquired resistance (SAR) which is mediated by the signaling molecules azelaic acid (AzA), glycerol-3-phosphate (G3P), and salicylic acid (SA). Negative regulator of plasmodesmata permeability triggered by SA during immune responses, through regulation of callose deposition. Delays the trafficking of Tobacco Mosaic Virus (TMV) movement protein (MP). Required for symplastic signal transport.
Gene References into Functions
  1. PDLP5 plays role in systemic acquired resistance and the long-distance movement of DIR1 protein in Arabidopsis. PMID: 25296648
  2. Overexpression of PDLP5 induces spontaneous cell death and chlorosis. PDLP5 modulates both basal and induced plasmodesmata permeability. It is linked to innate immunity against bacterial pathogens. PMID: 21934146
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell junction, plasmodesma. Note=Located in cell wall junctions between leaf epidermal and mesophyl cells. Located to the central region of plasmodesmata. Co-localizes with the Grapevine fanleaf virus (GFLV) 2B-MP at the base of tubules within modified plasmodesmata.
Protein Families
Cysteine-rich repeat secretory protein family, Plasmodesmata-located proteins (PDLD) subfamily
Tissue Specificity
Highly expressed in inflorescence nodes and rosette senescent leaves. Mostly expressed in cell wall junctions between leaf epidermal and mesophyl cells, and to a lesser extent at the cross walls between epidermal or cortex cells within the hypocotyl (at p
Database Links

KEGG: ath:AT1G70690

STRING: 3702.AT1G70690.1

UniGene: At.42976

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