Recombinant Arabidopsis thaliana Cytochrome P450 82C4 (CYP82C4), partial

Code CSB-YP886824DOA
MSDS
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Source Yeast
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Code CSB-EP886824DOA
MSDS
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Source E.coli
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Code CSB-EP886824DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP886824DOA
MSDS
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Source Baculovirus
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Code CSB-MP886824DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP82C4
Uniprot No.
Alternative Names
CYP82C4; At4g31940; F10N7.250Xanthotoxin 5-hydroxylase CYP82C4; 8-methoxypsoralen 5-hydroxylase CYP82C4; EC 1.14.14.-; Cytochrome P450 82C4; Fraxetin 5-hydroxylase CYP82C4; EC 1.14.14.164
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Can hydroxylate xanthotoxin (8-methoxypsoralen) to form 5-hydroxyxanthotoxin (5-hydroxy-8-methoxypsoralen) in vivo and in vitro. Involved in the early iron deficiency response, possibly through an IDE1-like mediated pathway. Involved in the pathway of sideretin biosynthesis from feruloyl CoA, a redox-active catecholic metabolite exuded by roots in response to iron deficiency in order to facilitate the uptake of iron; this pathway consists in the successive conversion from feruloyl CoA to scopoletin, from scopoletin to fraxetin and from fraxetin to sideretin. Catalyzes the biosynthesis of sideretin via fraxetin hydroxylation.
Gene References into Functions
  1. CYP82C4 (cytochrome P450, family 82, subfamily C, polypeptide 4 ) expression correlates with expression of genes involved in metal uptake and transport. PMID: 21315474
Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
Expressed in both primary and lateral roots under iron-deficient conditions, except in apical root zones, and mostly in the root epidermal layer.
Database Links

KEGG: ath:AT4G31940

STRING: 3702.AT4G31940.1

UniGene: At.31700

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