Recombinant Arabidopsis thaliana Cytochrome P450 82G1 (CYP82G1), partial

Code CSB-YP862869DOA
MSDS
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Source Yeast
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Code CSB-EP862869DOA
MSDS
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Source E.coli
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Code CSB-EP862869DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862869DOA
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Source Baculovirus
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Code CSB-MP862869DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP82G1
Uniprot No.
Alternative Names
CYP82G1; OR9; At3g25180; MJL12.5Dimethylnonatriene synthase; EC 1.14.14.59; Cytochrome P450 82G1; Protein OVIPOSITION RATE 9; Trimethyltridecatetraene synthase; EC 1.14.14.58
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the biosynthesis of homoterpenes, attractants of herbivores parasitoids and predators (e.g. predatory mites and parasitoid wasps). Catalyzes the conversion of the C20 (E,E)-geranyllinalool to C16-homoterpene 4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT) of the C15 (E)-nerolidol to C11-homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT); these volatile compounds are produced upon insect herbivore attack and emitted from flowers and vegetative tissues during herbivore feeding. Required during resistance responses to the fungus Alternaria brassicae. Prevents oviposition of the phloem-feeding insect cabbage whitefly (Aleyrodes proletella).
Gene References into Functions
  1. CYP82G1 is constitutively expressed in Arabidopsis stems and inflorescences and shows highly coordinated herbivore-induced expression with geranyllinalool synthase in leaves depending on the F-box protein COI-1. PMID: 21088219
Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
Expressed in stems, flower peduncles, receptacle of developing and mature flowers and in stigma of mature opening flower buds.
Database Links

KEGG: ath:AT3G25180

STRING: 3702.AT3G25180.1

UniGene: At.37412

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