Recombinant Arabidopsis thaliana Cytosolic sulfotransferase 12 (SOT12)

Code CSB-YP347147DOA
MSDS
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Source Yeast
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Code CSB-EP347147DOA
MSDS
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Source E.coli
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Code CSB-EP347147DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP347147DOA
MSDS
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Source Baculovirus
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Code CSB-MP347147DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SOT12
Uniprot No.
Alternative Names
SOT12; RAR047; ST1; At2g03760; F19B11.21; Cytosolic sulfotransferase 12; AtSOT12; EC 2.8.2.-; Sulfotransferase 1; AtST1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-326
Target Protein Sequence
MSSSSSVPAY LGDEDLTQET RALISSLPKE KGWLVSEIYE FQGLWHTQAI LQGILICQKR FEAKDSDIIL VTNPKSGTTW LKALVFALLN RHKFPVSSSG NHPLLVTNPH LLVPFLEGVY YESPDFDFSS LPSPRLMNTH ISHLSLPESV KSSSCKIVYC CRNPKDMFVS LWHFGKKLAP EETADYPIEK AVEAFCEGKF IGGPFWDHIL EYWYASRENP NKVLFVTYEE LKKQTEVEMK RIAEFLECGF IEEEEVREIV KLCSFESLSN LEVNKEGKLP NGIETKTFFR KGEIGGWRDT LSESLAEEID RTIEEKFKGS GLKFSS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the stereospecific sulfate conjugation of 24-epibrassinosteroids. Preferred substrates are 24-epicathasterone and 6-deoxo-24-epicathasterone. Low activity with 22-deoxy-24-epiteasterone. No activity with 24-epimers catasterone and brassinolide. Sulfonates salicylic acid. May be involved in detoxification. Enhances plant response to pathogen infection and contributes to long distance signaling in systemic acquired resistance (SAR).
Gene References into Functions
  1. AtSOT12 could sulphonate the bacterial-produced toxin cycloheximide. PMID: 25736839
  2. Data indicate tht 7-hydroxyflavone was sulfated by all three enzymes AtSULT202E1 (At2g03770), AtSULT202B1 (At3g45070), and AtSULT202A1 (At2g03760). PMID: 23611783
  3. Salycylic acid can be sulfonated by SOT12. Over-expressing plants are more resistant to pathogen infection. PMID: 20374532
  4. crystal structure of At2g03760; it has high structural similarity to two human steroid sulfotransferases: human estrogen sulfotransferase and dehydroepiandrosterone sulfotransferase PMID: 15317023
Subcellular Location
Cytoplasm.
Protein Families
Sulfotransferase 1 family
Tissue Specificity
Expressed in the aerial parts of seedlings, in roots, leaves and flowers. Not detected in stems and siliques.
Database Links

KEGG: ath:AT2G03760

STRING: 3702.AT2G03760.1

UniGene: At.400

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