Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 10 (RH10)

Code CSB-YP808426DOA
MSDS
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Source Yeast
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Code CSB-EP808426DOA
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Source E.coli
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Code CSB-EP808426DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP808426DOA
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Source Baculovirus
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Code CSB-MP808426DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RH10
Uniprot No.
Alternative Names
RH10; EAST2; At5g60990; MSL3.13; MSL3_110; DEAD-box ATP-dependent RNA helicase 10; EC 3.6.4.13; Protein ENHANCER OF ASYMMETRIC LEAVES TWO
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-456
Target Protein Sequence
MEEENEVVKT FAELGVREEL VKACERLGWK NPSKIQAEAL PFALEGKDVI GLAQTGSGKT GAFAIPILQA LLEYVYDSEP KKGRRPDPAF FACVLSPTRE LAIQIAEQFE ALGADISLRC AVLVGGIDRM QQTIALGKRP HVIVATPGRL WDHMSDTKGF SLKSLKYLVL DEADRLLNED FEKSLNQILE EIPLERKTFL FSATMTKKVR KLQRACLRNP VKIEAASKYS TVDTLKQQYR FVAAKYKDCY LVYILSEMPE STSMIFTRTC DGTRFLALVL RSLGFRAIPI SGQMTQSKRL GALNKFKAGE CNILVCTDVA SRGLDIPSVD VVINYDIPTN SKDYIHRVGR TARAGRSGVG ISLVNQYELE WYIQIEKLIG KKLPEYPAEE DEVLSLLERV AEAKKLSAMN MKESGGRKRR GEDDEESERF LGGNKDRGNK ERGGNKDKKS SKKFKR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in leaf polarity establishment by functioning cooperatively with AS2 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures. Involved in the processing of pre-rRNA intermediates at high temperatures.
Gene References into Functions
  1. RH10 maintains cytosine methylations at specific sets of CpG sites in exon 6 of ARF3.[RH10] PMID: 29415182
Subcellular Location
Nucleus. Nucleus, nucleolus.
Protein Families
DEAD box helicase family, DDX47/RRP3 subfamily
Tissue Specificity
Expressed in all tissues and organs examined including root, cotyledon, first and second leaves, third and fourth leaves, fifth and sixth leaves, shoot apex, flower, flower bud, cauline leaf and rosette leaves.
Database Links

KEGG: ath:AT5G60990

STRING: 3702.AT5G60990.1

UniGene: At.20430

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