Recombinant Arabidopsis thaliana Defensin-like protein 16 (PDF1.2A)

Code CSB-EP887761DOA
MSDS
Size $554
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
PDF1.2A
Uniprot No.
Research Area
others
Alternative Names
Low-molecular-weight cysteine-rich protein 77
Species
Arabidopsis thaliana (Mouse-ear cress)
Source
E.coli
Expression Region
30-80aa
Target Protein Sequence
QKLCEKPSGTWSGVCGNSNACKNQCINLEGAKHGSCNYVFPAHKCICYVPC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
5.5 kDa
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
13-23 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Confers broad-spectrum resistance to pathogens. Has antifungal activity in vitro.
Gene References into Functions
  1. Natural variation in jasmonic acid-induced PDF1.2 expression is associated with basal resistance against the nectrophic fungus Plectosphaerella cucumerina. PMID: 21414016
  2. ORA59 and two functionally equivalent GCC box binding sites form the module that enables the PDF1.2 gene to respond synergistically to simultaneous activation of the jasmonate and ethylene signaling pathways PMID: 21246258
  3. Growth and infection of Phakopsora pachyrhizi hyphae in Arabidopsis were terminated at the mesophyll boundary, and associated with expression of PDF1.2. PMID: 18842092
Subcellular Location
Secreted.
Protein Families
DEFL family
Tissue Specificity
Predominantly expressed in leaves.
Database Links

KEGG: ath:AT5G44420

STRING: 3702.AT5G44420.1

UniGene: At.27168

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