Recombinant Arabidopsis thaliana Dehydration-responsive element-binding protein 1A (DREB1A)

Code CSB-YP878658DOA
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Source Yeast
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Code CSB-EP878658DOA
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Source E.coli
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Code CSB-EP878658DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP878658DOA
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Source Baculovirus
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Code CSB-MP878658DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DREB1A
Uniprot No.
Alternative Names
DREB1A; CBF3; CRAP2; ERF072; At4g25480; M7J2.150Dehydration-responsive element-binding protein 1A; Protein DREB1A; C-repeat/dehydration-responsive element-binding factor 3; C-repeat-binding factor 3; CRT/DRE-binding factor 3; Cold resistance-related AP2 transcription factor
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-216
Target Protein Sequence
MNSFSAFSEM FGSDYESSVS SGGDYIPTLA SSCPKKPAGR KKFRETRHPI YRGVRRRNSG KWVCEVREPN KKTRIWLGTF QTAEMAARAH DVAALALRGR SACLNFADSA WRLRIPESTC AKDIQKAAAE AALAFQDEMC DATTDHGFDM EETLVEAIYT AEQSENAFYM HDEAMFEMPS LLANMAEGML LPLPSVQWNH NHEVDGDDDD VSLWSY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates cold-inducible transcription. CBF/DREB1 factors play a key role in freezing tolerance and cold acclimation.
Gene References into Functions
  1. The Arabidopsis thaliana gene DREB1A/CBF3, encoding a stress-inducible transcription factor. Ectopic expression of AtDREB1A in Salvia miltiorrhiza resulted in increased drought tolerance and increased photosynthetic rate when subjected to drought stress. PMID: 27485523
  2. These results indicate the implicit influence of rd29A::DREB1A on mechanisms underlying water uptake, stomatal response, transpiration efficiency and rooting architecture in water-stressed plants. PMID: 25326370
  3. Potato plants ectopically expressing AtCBF3 exhibited enhanced tolerance to high temperature, which is associated with improved photosynthesis and antioxidant defence via induction of the expression of many stress-inducible genes. PMID: 24811248
  4. The physiological studies revealed that the expression of AtDREB1A was associated with an increased accumulation of the osmotic substance proline, maintenance of chlorophyll, increased relative water content and decreased ion leakage under drought stress. PMID: 24398893
  5. Stress-inducible over-expression of Arabidopsis CBF3 gene may have the potential to enhance abiotic stress tolerance in oat. PMID: 22325896
  6. The results suggested that AtDREB1A could cause dwarfism mediated by GA biosynthesis pathway in soybean. PMID: 23029105
  7. Overexpression of AtDREB1A in soybean appears to enhance drought tolerance. PMID: 22033903
  8. SOC1 Directly Represses the Expression of CBF3 Genes. PMID: 19825833
  9. The recombinant DREB1A protein bound to A/GCCGACNT more efficiently than to A/GCCGACNA/G/C. PMID: 15165189
  10. Makes transgenic plants more tolerant to stress conditions. PMID: 15834008
  11. Regulon genes repressed by siz1 did not affect expression of ICE1, which encodes a MYC transcription factor that controls CBF3/DREB1A. PMID: 17416732
  12. The freezing tolerance of 38 independent transgenic potato lines was tested in vitro using plantlets transgenic for the DREB1A gene under control of the rd29A promoter. PMID: 17453213
  13. Overexpression of DREB1A improves stress tolerance to both freezing and dehydration while overexpression of an active form of DREB2A results in significant stress tolerance to dehydration but only slight tolerance to freezing. PMID: 19502356

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Database Links

KEGG: ath:AT4G25480

STRING: 3702.AT4G25480.1

UniGene: At.231

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