Recombinant Arabidopsis thaliana Dehydration-responsive element-binding protein 2A (DREB2A)

Code CSB-YP525747DOA
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Source Yeast
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Code CSB-EP525747DOA
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Source E.coli
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Code CSB-EP525747DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP525747DOA
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Source Baculovirus
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Code CSB-MP525747DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DREB2A
Uniprot No.
Alternative Names
DREB2A; ERF045; At5g05410; K18I23.22Dehydration-responsive element-binding protein 2A; Protein DREB2A
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-335
Target Protein Sequence
MAVYDQSGDR NRTQIDTSRK RKSRSRGDGT TVAERLKRWK EYNETVEEVS TKKRKVPAKG SKKGCMKGKG GPENSRCSFR GVRQRIWGKW VAEIREPNRG SRLWLGTFPT AQEAASAYDE AAKAMYGPLA RLNFPRSDAS EVTSTSSQSE VCTVETPGCV HVKTEDPDCE SKPFSGGVEP MYCLENGAEE MKRGVKADKH WLSEFEHNYW SDILKEKEKQ KEQGIVETCQ QQQQDSLSVA DYGWPNDVDQ SHLDSSDMFD VDELLRDLNG DDVFAGLNQD RYPGNSVANG SYRPESQQSG FDPLQSLNYG IPPFQLEGKD GNGFFDDLSY LDLEN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates high salinity- and dehydration-inducible transcription.
Gene References into Functions
  1. Genetic analysis indicated that the depletion of BTB/POZ AND MATH DOMAIN protein expression conferred enhanced thermotolerance via DREB2A stabilization PMID: 28923951
  2. DPB3-1 associates with NF-YA and NF-YB subunits to enhance heat stress-inducible gene expression during heat stress responses in cooperation with DREB2A. PMID: 25490919
  3. The stabilization of DREB2A is important but not sufficient to induce target gene expression; further activation processes are required. PMID: 24376497
  4. The stress-inducible expression of Arabidopsis thaliana DREB2A CA in transgenic sugarcane led to the up-regulation of genes involved in plant response to drought stress. PMID: 24656336
  5. GRF7 binds to a region of the DREB2A promoter and represses its activity. PMID: 22942381
  6. Functional interactions between Dreb2a and Med25 induce conformational changes which in turn cause changes in their binding affinity. PMID: 22447446
  7. In addition RCD1 is rapidly degraded during heat stress, thus our results suggest that removal of RCD1 protein or the loss of the interaction domain in DREB2A appears to be required for proper DREB2A function under stress conditions. PMID: 22150398
  8. These results suggest that in addition to an abscissic acid (ABA)-independent pathway, the ABA-dependent pathway plays a positive role in the osmotic stress-responsive expression of DREB2A. PMID: 22025559
  9. The region between DREB2A residues 136 and 165 plays a role in the stability of this protein in the nucleus, which is important for protein activation. PMID: 16617101
  10. Functions in both water-stress and heat shock-stress responses. PMID: 17030801
  11. Altogether these data demonstrate that HsfA3 is transcriptionally controlled by DREB2A and important for the establishment of thermotolerance. PMID: 17999647
  12. These results indicate that HsfA3 regulates expression of many heat-inducible genes in the transcriptional cascade downstream of the DREB2A stress-regulatory system and functions in acquisition of thermotolerance under the control of the DREB2A cascade. PMID: 18261981

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity
Expressed preferentially in roots and stems, and at a lower level in leaves.
Database Links

KEGG: ath:AT5G05410

STRING: 3702.AT5G05410.1

UniGene: At.25247

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