Product Details

Purity

>85% (SDS-PAGE)

Target Names

DCL4

Uniprot No.

P84634

Alternative Names

DCL4; At5g20320; F5O24.210Dicer-like protein 4; EC 3.1.26.-

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the biogenesis of trans-acting small interfering RNAs (ta-siRNAs, derived from the TAS1, TAS2 or TAS3 endogenous transcripts) by cleaving small dsRNAs into 21-24 nucleotide ta-siRNAs. Functions with the dsRNA-binding protein DRB4 in ta-siRNAs processing. Acts in the RDR6/SGS3/DCL4/AGO7 ta-siRNA pathway involved in leaf developmental timing. Plays a role in transitive silencing of transgenes by processing secondary siRNAs. This pathway, which requires DCL2 and RDR6, amplifies silencing by using the target RNA as substrate to generate secondary siRNAs, providing an efficient mechanism for long-distance silencing. Required for the production of the 30-40 nucleotide bacterial-induced long siRNAs (lsiRNA). May participate with DCL3 in the production of 24 nucleotide repeat-associated siRNAs (ra-siRNAs) which derive from heterochromatin and DNA repeats such as transposons. Plays an important role in antiviral RNA silencing. Involved in the production of viral siRNAs derived from the cucumber mosaic virus (CMV), turnip crinkle virus (TCV) and tobacco rattle virus (TRV). Targeted by the viral silencing suppressor (VSR) protein 2b of the cucumber mosaic virus (CMV) that inactivates DCL4 function in RNA silencing. Does not seem to be involved in microRNAs (miRNAs) processing.

Gene References into Functions

results suggest that the spatial orientation of the Connector helix, Platform and PAZ relative to the RNase III domains is crucial for measuring dsRNA of defined length PMID: 27907832
DCL4 cleaves long dsRNAs with blunt ends or with a 1 or 2 nt 3' overhang with similar efficiency. PMID: 24214956
DCL1 and DCL4 may cooperatively process TAS (trans-acting small interfering RNAs) transcripts and produce siRNAs that are required for DNA methylation and targeting mRNA cleavage. PMID: 22846193
DRB4 is specifically required for DCL4 activity in vitro. PMID: 21270136
Study reports the structure of the DUF283 domain from the Arabidopsis thaliana DCL4; the DUF283 domain adopts an alpha-beta-beta-beta-alpha topology and resembles the structural similarity to the double-stranded RNA-binding domain. PMID: 20106953
Studies indicate that by converting a single, cell-autonomous miRNA species into many DCL4-dependent siRNAs might increase the miRNA activity-range over several cell layers. PMID: 19822452
DCL4 is the primary processor of endogenous RDR6-dependent trans-acting siRNAs (tasiRNAs). PMID: 16040244
dcl4-2 mutant resembled an RNA-dependent RNA polymerase 6 mutant in that both mutants lacked ta-small interfering RNAs [DCL4] PMID: 16129836
DICER-LIKE 4 (DCL4) produces the 21-nt siRNA from the inverted repeat transgenes is the RNA component of cell-to-cell silencing signal in plants. [DCL4] PMID: 16273107
DCL4 is the primary producer of Cucumber mosaic virus (CMV) derived siRNAs and also produces turnip crinkle virus (TCV)-derived siRNAs. DCL2 appears to be the major producer of TCV-derived siRNAs, likely because TCV suppresses the activity of DCL4.[DCL4] PMID: 16810317
DCL4 is capable of accessing flexibly structured single-stranded RNA substrates (preferably T-shaped hairpins) to produce satsiRNAs. This result reveals that viral RNA of diverse structures may stimulate antiviral DCL activities in plant cells. PMID: 17609283
DCL1 represses antiviral RNA silencing through negatively regulating the expression of DCL4 and DCL3 PMID: 18799732

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Subcellular Location

Nucleus.

Protein Families

Helicase family, Dicer subfamily

Database Links

KEGG: ath:AT5G20320

STRING: 3702.AT5G20320.1

UniGene: At.31201

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Recombinant Arabidopsis thaliana Dicer-like protein 4 (DCL4), partial

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