Recombinant Arabidopsis thaliana Disease resistance protein RPP8 (RPP8), partial

Code CSB-YP855418DOA
MSDS
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Source Yeast
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Code CSB-EP855418DOA
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Source E.coli
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Code CSB-EP855418DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP855418DOA
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Source Baculovirus
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Code CSB-MP855418DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RPP8
Uniprot No.
Alternative Names
RPP8; HRT; At5g43470; MWF20.19; Disease resistance protein RPP8; Resistance to Peronospora parasitica protein 8
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Disease resistance protein. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. The interaction with TIP (TCV-interacting protein) may be essential for the recognition of the avirulence proteins, and the triggering of the defense response. Triggers resistance to turnip crinkle virus (TCV) via a SAG101-dependent pathway.
Gene References into Functions
  1. This study examined the regulation of RPP8 in response to Hyaloperonospora arabidopsidis and salicylic acid. PMID: 20831409
  2. HRT-turnip crinkle virus interaction is unable to generate sufficient amounts of salicylic acid required for a stable resistance phenotype. [HRT] PMID: 15546349
  3. The evolutionary history of RPP8 in A. thaliana was studied. PMID: 18182023
Subcellular Location
Cell membrane.
Protein Families
Disease resistance NB-LRR family, RPP8/HRT subfamily
Tissue Specificity
Mostly expressed in leaves, and, to a lower extent, in roots.
Database Links

KEGG: ath:AT5G43470

STRING: 3702.AT5G43470.1

UniGene: At.47555

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