Recombinant Arabidopsis thaliana Dof zinc finger protein DOF1.10 (DOF1.10)

Code CSB-YP871048DOA
MSDS
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Source Yeast
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Code CSB-EP871048DOA
MSDS
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Source E.coli
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Code CSB-EP871048DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871048DOA
MSDS
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Source Baculovirus
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Code CSB-MP871048DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CDF5
Uniprot No.
Alternative Names
CDF5; DOF1.10; HPPBF-2B; At1g69570; F10D13.20; F24J1.25Cyclic dof factor 5; Dof zinc finger protein DOF1.10; AtDOF1.10; H-protein promoter-binding factor 2b
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-399
Target Protein Sequence
MSKSRDTEIK LFGRTITSLL DVNCYDPSSL SPVHDVSSDP SKEDSSSSSS SCSPTIGPIR VPVKKSEQES NKFKDPYILS DLNEPPKAVS EISSPRSSKN NCDQQSEITT TTTTSTTSGE KSTALKKPDK LIPCPRCESA NTKFCYYNNY NVNQPRYFCR NCQRYWTAGG SMRNVPVGSG RRKNKGWPSS NHYLQVTSED CDNNNSGTIL SFGSSESSVT ETGKHQSGDT AKISADSVSQ ENKSYQGFLP PQVMLPNNSS PWPYQWSPTG PNASFYPVPF YWGCTVPIYP TSETSSCLGK RSRDQTEGRI NDTNTTITTT RARLVSESLR MNIEASKSAV WSKLPTKPEK KTQGFSLFNG FDTKGNSNRS SLVSETSHSL QANPAAMSRA MNFRESMQQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that binds specifically to a 5'-AA[AG]G-3' consensus core sequence. Regulates a photoperiodic flowering response. Transcriptional repressor of 'CONSTANS' expression.
Gene References into Functions
  1. CDF5/FLORE NAT pair constitutes an additional circadian regulatory module with conserved (mutual inhibition) and unique (function in trans) features, able to fine-tune its own circadian oscillation, and consequently, adjust the onset of flowering to favourable environmental conditions. [CDF5] PMID: 28758689
Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT1G69570

STRING: 3702.AT1G69570.1

UniGene: At.10732

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