Recombinant Arabidopsis thaliana Dynamin-like protein ARC5 (ARC5), partial

Code CSB-YP769562DOA
MSDS
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Source Yeast
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Code CSB-EP769562DOA
MSDS
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Source E.coli
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Code CSB-EP769562DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP769562DOA
MSDS
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Source Baculovirus
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Code CSB-MP769562DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARC5
Uniprot No.
Alternative Names
ARC5; DRP5B; At3g19720; MMB12.21Dynamin-like protein ARC5; EC 3.6.5.5; Dynamin-related protein 5B; Protein ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probable GTPase component of both plastid and peroxisme division machinery. Required for the last steps of plastid division specifically in mesophyll-cell, when the narrow isthmus breaks, facilitating the separation of the daughter plastids. Necessary for peroxisome activities. Seems to influence stromule (stroma-filled tubular extensions of the plastid envelope membrane) length and frequency.
Gene References into Functions
  1. results support the notion that ARC5 is dispensable in the process of equal division of epidermal plastids, and indicate that dysfunctions in ARC5 and ARC6 differentially affect plastid replication among mesophyll cells, PCs, and GCs within a single leaf PMID: 29466386
  2. ARC5, which encodes a cytosolic dynamin-like protein that is part of the chloroplast division machinery and arc mutants have large but fewer chloroplasts which have an effect on both the composition and structure of the photosynthetic apparatus. [ARC5] PMID: 16170638
  3. arc5 plastids appeared wild-type in the majority of nongreen tissues examined PMID: 18764889
Subcellular Location
Cytoplasm. Plastid, chloroplast outer membrane; Peripheral membrane protein. Peroxisome. Note=Plastid equatorial positioning (in a discontinuous ring shape) mediated by PDV2 in complex with ARC6 and by PDV1.
Protein Families
TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family
Database Links

KEGG: ath:AT3G19720

STRING: 3702.AT3G19720.1

UniGene: At.38361

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