Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase BAH1 (BAH1)

Code CSB-YP865959DOA
MSDS
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Source Yeast
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Code CSB-EP865959DOA
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Source E.coli
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Code CSB-EP865959DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865959DOA
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Source Baculovirus
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Code CSB-MP865959DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BAH1
Uniprot No.
Alternative Names
BAH1; NLA; At1g02860; F22D16.14E3 ubiquitin-protein ligase BAH1; EC 2.3.2.27; Protein BENZOIC ACID HYPERSENSITIVE 1; Protein NITROGEN LIMITATION ADAPTATION; RING-type E3 ubiquitin transferase BAH1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-335
Target Protein Sequence
MKFCKKYEEY MQGQKEKKNL PGVGFKKLKK ILKRCRRNHV PSRISFTDAI NHNCSRECPV CDGTFFPELL KEMEDVVGWF NEHAQKLLEL HLASGFTKCL TWLRGNSRKK DHHGLIQEGK DLVNYALINA VAIRKILKKY DKIHESRQGQ AFKTQVQKMR IEILQSPWLC ELMAFHINLK ESKKESGATI TSPPPPVHAL FDGCALTFDD GKPLLSCELS DSVKVDIDLT CSICLDTVFD PISLTCGHIY CYMCACSAAS VNVVDGLKTA EATEKCPLCR EDGVYKGAVH LDELNILLKR SCRDYWEERR KTERAERLQQ AKEYWDYQCR SFTGI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mediates E2-dependent protein ubiquitination. Plays a role in salicylic acid-mediated negative feedback regulation of salicylic acid (SA) accumulation. May be involved in the overall regulation of SA, benzoic acid and phenylpropanoid biosynthesis. Controls the adaptability to nitrogen limitation by channeling the phenylpropanoid metabolic flux to the induced anthocyanin synthesis.
Gene References into Functions
  1. BAH1, an E3 ligase from plant that has a similar zinc finger domain to DnaJ, can perform block the effect of DnaK on sigma(32) in Escherichia coli. PMID: 29260303
  2. NLA interacts with NRT1.7 in the plasma membrane. PMID: 28032637
  3. NLA is important for regulating the abundance of PHT1 proteins to maintain Pi homeostasis PMID: 24122828
  4. NLA and miR827 have pivotal roles in regulating Pi homeostasis in plants in a nitrate-dependent fashion PMID: 21455488
  5. Nitrogen limitation triggers extensive transcriptome response in Arabidopsis plants and is regulated by NLA. [NLA] PMID: 17885809
  6. controls Arabidopsis adaptability to nitrogen limitation by channelling the phenylpropanoid metabolic flux to the induced anthocyanin synthesis PMID: 18552353
  7. BAH1/NLA plays a crucial role in the ubiquitination-mediated regulation of immune response, and regulates the accumulation of salicylic acid. PMID: 18753285

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Subcellular Location
Nucleus speckle.
Tissue Specificity
High expression in roots and stems, medium in seedlings, flowers, rosette and cauline leaves, and very low in siliques. Detected in cotyledons, hypocotyls, pedicel, receptacle, pistil, sepal, filament of stamen and at the two ends of developing siliques.
Database Links

KEGG: ath:AT1G02860

STRING: 3702.AT1G02860.1

UniGene: At.22880

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