Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase RGLG1 (RGLG1)

Code CSB-YP890421DOA
MSDS
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Source Yeast
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Code CSB-EP890421DOA
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Source E.coli
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Code CSB-EP890421DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP890421DOA
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Source Baculovirus
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Code CSB-MP890421DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RGLG1
Uniprot No.
Alternative Names
RGLG1; At3g01650; F4P13.19; E3 ubiquitin-protein ligase RGLG1; EC 2.3.2.27; RING domain ligase 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
2-489
Target Protein Sequence
GGGNSKEES SSPSSSSWAS HQSYPQYGPD SYNYPPPPTY APAPSPAPAP APVPAPSPAS SYGPQYSQEG YASQPNNPPP PTYAPAPSPA SSYGHQYSQE GYASAASQPN YPPPPSQSQV ADRKKFDRRY SKISDNYSSL LQVSEALGRA GLESSNLIVG IDFTKSNEWT GAKSFNRKSL HHLSNTPNPY EQAITIIGRT LAAFDEDNLI PCYGFGDAST HDQDVFSFYP EGRFCNGFEE VLARYREIVP QLKLAGPTSF APIIEMAMTV VEQSSGQYHV LVIIADGQVT RSVDTEHGRL SPQEQKTVDA IVKASTLPLS IVLVGVGDGP WDMMQEFDDN IPARAFDNFQ FVNFTEIMSK NKDQSRKETE FALSALMEIP PQYKATIELN LLGVRNGNIP QRIPLPPPVQ SGSSFSSSRI PNFEPSVPPY PFESKQMSSA DDIQLCPICL SNPKNMAFGC GHQTCCECGP DLKVCPICRA PIQTRIKLY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin-protein ligase that mediates the formation of 'Lys-63'-linked ubiquitin chains. Regulates apical dominance by acting on the auxin transport proteins abundance. Together with RGLG5, mediates the ubiquitination and subsequent proteasomal degradation of the target protein PP2CA. Functions as positive regulator of abscisic acid (ABA) signaling through ABA-dependent degradation of PP2CA, a major inhibitor of ABA signaling. Acts as a negative regulator of drought stress response.
Gene References into Functions
  1. Post-Transcriptional Coordination of the Arabidopsis Iron Deficiency Response is Partially Dependent on the E3 Ligases RING DOMAIN LIGASE1 (RGLG1) and RING DOMAIN LIGASE2 (RGLG2 PMID: 26253232
Subcellular Location
Cell membrane; Lipid-anchor. Nucleus.
Tissue Specificity
Ubiquitously expressed.
Database Links

KEGG: ath:AT3G01650

STRING: 3702.AT3G01650.1

UniGene: At.22440

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